Morii E, Hirota S, Kim H M, Mikoshiba K, Nishimune Y, Kitamura Y, Nomura S
Department of Pathology, Osaka University Medical School, Suita, Japan.
Brain Res Dev Brain Res. 1992 Jan 17;65(1):123-6. doi: 10.1016/0165-3806(92)90016-p.
Expression of mRNA for c-kit receptors and their ligands was examined in the cerebellum of mice by in situ hybridization technique. The c-kit receptors were expressed in the molecular layer of the cerebellum and the ligands for the c-kit receptors were detected at the boundary of molecular and granular layers. The expression of the c-kit ligands was not detectable in the cerebellum of lurcher (Lc/+) mutant mice that lack Purkinje cells, indicating the cells expressing the c-kit ligands were Purkinje cells. The cells expressing c-kit receptors decreased but were present in the cerebellum of Lc/+ mice. The c-kit mRNA-positive cells appeared to represent basket cells and stellate cells from their appearance and location. Since neurons in the molecular layer construct suppressive neurojunctions with Purkinje cells, the present result suggests that c-kit receptors and their ligands may play an important role for the construction of their junctions.
采用原位杂交技术检测了小鼠小脑组织中c-kit受体及其配体的mRNA表达情况。c-kit受体在小脑分子层表达，c-kit受体的配体在分子层与颗粒层交界处被检测到。在缺乏浦肯野细胞的蹒跚（Lc/+）突变小鼠的小脑中未检测到c-kit配体的表达，这表明表达c-kit配体的细胞是浦肯野细胞。表达c-kit受体的细胞数量减少，但仍存在于Lc/+小鼠的小脑中。从c-kit mRNA阳性细胞的外观和位置来看，它们似乎代表篮状细胞和星状细胞。由于分子层中的神经元与浦肯野细胞构建抑制性神经连接，目前的结果表明c-kit受体及其配体可能在其连接的构建中发挥重要作用。
