Sudol M, Kuo C F, Shigemitsu L, Alvarez-Buylla A
Rockefeller University, New York, New York 10021.
Mol Cell Biol. 1989 Oct;9(10):4545-9. doi: 10.1128/mcb.9.10.4545-4549.1989.
To identify the kinds of cells in the brain that express the yes proto-oncogene, we examined chicken brains by using immunofluorescent staining and in situ hybridization. Both approaches showed that the highest level of the yes gene product was in cerebellar Purkinje cells. In addition, we analyzed Purkinje cell degeneration (pcd) mutant mice. The level of yes mRNA in cerebella of pcd mutants was four times lower than that found in cerebella of normal littermates. Our studies point to Purkinje cells as an attractive model for functional studies of the yes protein.
为了鉴定大脑中表达yes原癌基因的细胞类型，我们通过免疫荧光染色和原位杂交技术对鸡脑进行了检测。两种方法均显示，yes基因产物的最高水平存在于小脑浦肯野细胞中。此外，我们分析了浦肯野细胞变性（pcd）突变小鼠。pcd突变小鼠小脑内yes mRNA的水平比正常同窝小鼠小脑内的水平低四倍。我们的研究表明，浦肯野细胞是对yes蛋白进行功能研究的一个有吸引力的模型。
