采用人乳头瘤病毒寡核苷酸微阵列检测不同宫颈病变中的人乳头瘤病毒基因型并进行分型

Detection and typing of HPV genotypes in various cervical lesions by HPV oligonucleotide microarray.

作者信息

Hwang Tae Sook, Jeong Jeongmi Kim, Park Misun, Han Hye Seung, Choi Hyo Kyoung, Park Tae Shin

机构信息

Department of Pathology, Inha University College of Medicine, Incheon 402-712, South Korea.

出版信息

Gynecol Oncol. 2003 Jul;90(1):51-6. doi: 10.1016/s0090-8258(03)00201-4.

DOI:10.1016/s0090-8258(03)00201-4

PMID:12821341

Abstract

OBJECTIVES

This study was conducted to evaluate a clinical efficacy of human papillomavirus (HPV) oligonucleotide microarray (Biomedlab Co., Seoul, South Korea) for the detection of HPVs in various cervical lesions.

RESULTS

HPV DNAs from 234 patients were analysed by two methods. Among those, 212 patients were classified into 5 groups according to the histologic diagnosis: chronic cervicitis, cervical intraepithelial neoplasia (CIN) I, CIN II, CIN III, and invasive cervical carcinoma. PCR-RFLP could detect 7 types of high-risk HPVs (HPV 16/18/31/33/35/52/58) and HPV microarray could detect 15 types of high-risk HPVs (HPV 16/18/31/33/35/39/45/51/52/56/58/59/66/68/69) and 7 types of low-risk HPVs (HPV 6/11/34/40/42/43/44).HPV genotyping by HPV oligonucleotide microarray revealed that HPV16 was the most frequent type (30.2%) in all specimens tested and was significantly more frequent in CIN III and invasive carcinomas than other lesions.

METHODS

HPV DNAs were detected in 158 and 174 of the 234 cervical samples by PCR-RFLP and HPV microarray, respectively. The correlation between the two methods was good in detecting HPVs in general (kappa index = 0.69) and HPVs 31 and 52 (kappa index = 0.70 and 0.70, respectively) and excellent in detecting HPVs 16, 18, 33, 35, and 58 (kappa index = 0.90, 0.88, 0.92, 0.77, and 0.84, respectively). Double HPV infection was detected in 10 cases and one triple infection was detected. By combining cytology and HPV testing, the sensitivity was improved to 87.5, 95.5, 96.1, and 97.2% in CIN I, CIN II, CIN III, and carcinoma, respectively.

CONCLUSIONS

This results suggest that HPV oligonucleotide microarray is a highly comparable method to the previously used PCR-RFLP method for the detection of HPVs in cervical specimens. Genetic informations for HPV infection in cervical specimens may offer new strategies in manipulating the patients harboring cervical intraepithelial neoplasia and cervical carcinoma.

摘要

目的

本研究旨在评估人乳头瘤病毒（HPV）寡核苷酸微阵列（韩国首尔Biomedlab公司）检测各种宫颈病变中HPV的临床疗效。

结果

采用两种方法对234例患者的HPV DNA进行分析。其中，212例患者根据组织学诊断分为5组：慢性宫颈炎、宫颈上皮内瘤变（CIN）I级、CIN II级、CIN III级和浸润性宫颈癌。聚合酶链反应-限制性片段长度多态性（PCR-RFLP）可检测7种高危型HPV（HPV 16/18/31/33/35/52/58），HPV微阵列可检测15种高危型HPV（HPV 16/18/31/33/35/39/45/51/52/56/58/59/66/68/69）和7种低危型HPV（HPV 6/11/34/40/42/43/44）。HPV寡核苷酸微阵列基因分型显示，HPV16是所有检测标本中最常见的类型（30.2%），在CIN III级和浸润性癌中的出现频率显著高于其他病变。

方法

分别采用PCR-RFLP和HPV微阵列在234份宫颈样本中的158份和174份中检测到HPV DNA。两种方法在检测HPV方面总体相关性良好（kappa指数=0.69），在检测HPV 31和52时相关性良好（kappa指数分别为0.70和0.70），在检测HPV 16、18、33、35和58时相关性极佳（kappa指数分别为0.90、0.88、0.92、0.77和0.84）。检测到10例双重HPV感染和1例三重感染。联合细胞学和HPV检测，CIN I级、CIN II级、CIN III级和癌的敏感性分别提高到87.5%、95.5%、96.1%和97.2%。