Zheng Jian
Department of Gynecology and Obstetrics, Affiliated Hospital of Inner Mongolia Medical University 1 Channel North Road, Huimin District, Hohhot City, Inner Mongolia Autonomous Region, P. R. China.
Int J Clin Exp Pathol. 2015 Jan 1;8(1):875-80. eCollection 2015.
Cervical cancer remains the fourth most common cause of cancer-related deaths in women worldwide, and human papillomavirus infection represents the most important risk factor for the development of cervical cancer. Minichromosome maintenance protein-2 has been previously identified by DNA microarray and transcriptional profiling as genes that is overexpressed in cervical carcinomas. 183 cases were enrolled and tested with thin prep liquid-based cytology test. The expressions of human papillomavirus were detected and minichromosome maintenance protein-2 immuncytochemical test was performed on liquid-based pap smears from the samples. Those results were compared with the cervical histopathology results. The positive expression rates of minichromosome maintenance protein-2 and high-risk type human papillomavirus increased with the severity of cervical lesions. The expression level of MCM2 was positively correlated with high-risk types of human papillomavirus. In cervical carcinoma and precancerous lesions, minichromosome maintenance protein-2 was overexpressed and positively correlated with the high risk types of human papillomavirus. As minichromosome maintenance protein-2 immuncytochemical detection was better than genotyping of human papillomavirus, minichromosome maintenance protein-2 may serve as a useful marker in the screening of cervical carcinoma and precancerous lesions and improve the diagnosis of atypical squamous cell of undetermined significance. The joint application can improve the sensitivity and specificity of diagnosis.
宫颈癌仍是全球女性癌症相关死亡的第四大常见原因，人乳头瘤病毒感染是宫颈癌发生的最重要风险因素。微小染色体维持蛋白2先前已通过DNA微阵列和转录谱分析被鉴定为在宫颈癌中过度表达的基因。纳入183例患者并进行薄层液基细胞学检测。检测人乳头瘤病毒的表达，并对样本的液基巴氏涂片进行微小染色体维持蛋白2免疫细胞化学检测。将这些结果与宫颈组织病理学结果进行比较。微小染色体维持蛋白2和高危型人乳头瘤病毒的阳性表达率随宫颈病变严重程度增加而升高。MCM2的表达水平与高危型人乳头瘤病毒呈正相关。在宫颈癌和癌前病变中，微小染色体维持蛋白2过度表达且与高危型人乳头瘤病毒呈正相关。由于微小染色体维持蛋白2免疫细胞化学检测优于人乳头瘤病毒基因分型，微小染色体维持蛋白2可作为宫颈癌和癌前病变筛查的有用标志物，并改善意义不明确的非典型鳞状细胞的诊断。联合应用可提高诊断的敏感性和特异性。
