草绿色链球菌是拓扑异构酶IV基因向肺炎链球菌进行水平转移的供体。
Viridans group streptococci are donors in horizontal transfer of topoisomerase IV genes to Streptococcus pneumoniae.
作者信息
Balsalobre Luz, Ferrándiz María José, Liñares Josefina, Tubau Fe, de la Campa Adela G
机构信息
Unidad de Genética Bacteriana (Consejo Superior de Investigaciones Científicas), Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain.
出版信息
Antimicrob Agents Chemother. 2003 Jul;47(7):2072-81. doi: 10.1128/AAC.47.7.2072-2081.2003.
A total of 46 ciprofloxacin-resistant (Cip(r)) Streptococcus pneumoniae strains were isolated from 1991 to 2001 at the Hospital of Bellvitge. Five of these strains showed unexpectedly high rates of nucleotide variations in the quinolone resistance-determining regions (QRDRs) of their parC, parE, and gyrA genes. The nucleotide sequence of the full-length parC, parE, and gyrA genes of one of these isolates revealed a mosaic structure compatible with an interspecific recombination origin. Southern blot analysis and nucleotide sequence determinations showed the presence of an ant-like gene in the intergenic parE-parC regions of the S. pneumoniae Cip(r) isolates with high rates of variations in their parE and parC QRDRs. The ant-like gene was absent from typical S. pneumoniae strains, whereas it was present in the intergenic parE-parC regions of the viridans group streptococci (Streptococcus mitis and Streptococcus oralis). These results suggest that the viridans group streptococci are acting as donors in the horizontal transfer of fluoroquinolone resistance genes to S. pneumoniae.
1991年至2001年期间，在贝尔维奇医院共分离出46株耐环丙沙星的肺炎链球菌菌株（Cip(r)）。其中5株菌株在其parC、parE和gyrA基因的喹诺酮耐药决定区（QRDRs）显示出意外高的核苷酸变异率。对其中一株分离株的全长parC、parE和gyrA基因进行核苷酸序列分析，发现其具有与种间重组起源相符的嵌合结构。Southern印迹分析和核苷酸序列测定表明，在parE和parC QRDRs变异率高的肺炎链球菌Cip(r)分离株的parE-parC基因间隔区存在一个类ant基因。典型肺炎链球菌菌株中不存在类ant基因，而在草绿色链球菌（缓症链球菌和口腔链球菌）的parE-parC基因间隔区存在该基因。这些结果表明，草绿色链球菌在氟喹诺酮耐药基因向肺炎链球菌的水平转移中充当供体。
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