人胎盘醛糖还原酶催化的氢转移的立体特异性
Stereospecificity of the hydrogen transfer catalyzed by human placental aldose reductase.
作者信息
Feldman H B, Szczepanik P A, Havre P, Corrall R J, Yu L C, Rodman H M, Rosner B A, Klein P D, Landau B R
出版信息
Biochim Biophys Acta. 1977 Jan 11;480(1):14-20. doi: 10.1016/0005-2744(77)90316-3.
Placental aldose reductase (EC 1.1.1.21) was incubated with glucose in the presence of [4A-2H] NADPH prepared in the oxidation of [2-2H] isocitrate by isocitrate dehydrogenase (EC 1.1.1.42) or [4B-2H] NADPH prepared in the oxidation of [1-2H] glucose-6-phosphate dehydrogenase (EC 1.1.1.49). The sorbitol formed from [4A-2H] NADPH contained deuterium and from [4B-2H] NADPH it did not. Therefore, aldose reductase in an A-type enzyme.
胎盘醛糖还原酶（EC 1.1.1.21）与葡萄糖在异柠檬酸脱氢酶（EC 1.1.1.42）氧化[2-2H]异柠檬酸制备的[4A-2H]NADPH或葡萄糖-6-磷酸脱氢酶（EC 1.1.1.49）氧化[1-2H]葡萄糖制备的[4B-2H]NADPH存在的情况下一起孵育。由[4A-2H]NADPH形成的山梨醇含有氘，而由[4B-2H]NADPH形成的山梨醇则不含氘。因此，醛糖还原酶是一种A型酶。
Zotero 插件