Uda A, Tanabayashi K, Mukai R, Terao K, Yamada A
Tsukuba Primate Center for Medical Science, National Institute of Infectious Diseases, Ibaraki, Japan.
J Med Primatol. 2003 Apr;32(2):105-10. doi: 10.1034/j.1600-0684.2003.00012.x.
The FN18 monoclonal antibody (mAb), directed to CD3 molecules, did not react with the lymphocytes of some cynomolgus monkeys (Macaca fascicularis), because of the polymorphism of the CD3epsilon chain. The epitope recognized by the FN18 mAb was successfully expressed on COS7 cells upon transfection of plasmid DNA coding for the CD3epsilon derived from T cells of a FN18 positive cynomolgus monkey. By construction and expression of plasmid DNA encoding the mutant CD3epsilon, the amino acid residue at position 67 was demonstrated to be involved in the formation of an epitope recognizable by the FN18 mAb.
针对CD3分子的FN18单克隆抗体（mAb），由于CD3ε链的多态性，不与某些食蟹猴（猕猴）的淋巴细胞发生反应。当转染编码来自FN18阳性食蟹猴T细胞的CD3ε的质粒DNA时，FN18 mAb识别的表位在COS7细胞上成功表达。通过构建和表达编码突变型CD3ε的质粒DNA，证明第67位氨基酸残基参与了可被FN18 mAb识别的表位的形成。
