Gaur L K, Heise E R, Hansen J A, Clark E A
Regional Primate Research Center, University of Washington, Seattle 98195.
Immunogenetics. 1988;27(5):356-62. doi: 10.1007/BF00395131.
Fifty mouse monoclonal antibodies (mAb) specific for HLA class I epitopes were compared for their reactivity against two closely related nonhuman primate species, pigtailed macaques (Macaca nemestrina, Mn) and longtailed macaques (M. fascicularis, Mfl), which diverged from the hominoids 23-40 million years ago. An analysis of Nei's genetic identity (I) and distance (D) based on reactivity of all class I-specific mAb showed, as expected, that the macaques are more closely related to each other (I = 0.959) than to man (I = 0.782 for Mn and 0.859 for Mfl). However, there were clear differences in genetic similarity with respect to certain epitopes. Macaques were most different from each other and from man in expression of heterologous epitopes recognized by the mouse that are not polymorphic among humans. In contrast, the most polymorphic epitopes unique to single HLA alleles, so-called private epitopes, were present in all the species, and neither macaque species could be distinguished from humans, suggesting that certain class I private epitopes may be highly conserved in evolution.
针对50种对HLA I类表位具有特异性的小鼠单克隆抗体（mAb），比较了它们对两种亲缘关系密切的非人灵长类动物的反应性，即猪尾猕猴（食蟹猴，Mn）和长尾猕猴（M. fascicularis，Mfl），这两种猕猴在2300万至4000万年前与类人猿分化。基于所有I类特异性mAb的反应性对Nei氏遗传同一性（I）和距离（D）进行分析，正如预期的那样，结果显示猕猴彼此之间的亲缘关系（I = 0.959）比与人类的亲缘关系更近（Mn与人类的I = 0.782，Mfl与人类的I = 0.859）。然而，在某些表位的遗传相似性方面存在明显差异。在表达小鼠识别的非多态性异源表位方面，猕猴彼此之间以及与人类之间差异最大。相比之下，所有物种中都存在单HLA等位基因特有的最多态性表位，即所谓的私有表位，并且无法区分任何一种猕猴与人类，这表明某些I类私有表位在进化过程中可能高度保守。
