一种识别牛T细胞受体复合物CD3ε链的单克隆抗体的特性分析。
Characterisation of a monoclonal antibody recognising the CD3epsilon chain of the bovine T cell receptor complex.
作者信息
MacHugh N D, Mburu J K, Hamilton M J, Davis W C
机构信息
International Livestock Research Institute, Nairobi, Kenya.
出版信息
Vet Immunol Immunopathol. 1998 Feb 16;61(1):25-35. doi: 10.1016/s0165-2427(97)00087-1.
We describe the characterisation of a monoclonal antibody (mAb), designated MM1A, which reacts with an antigen molecule on the surface of bovine alphabeta and gammadeltaTcR+ T cells. The mAb immunoprecipitated a series of polypeptides of 21 kDa, 22 kDa, 32 kDa, 36 kDa and 44 kDa which is consistent with it recognising the TcR/CD3 complex. COS cells, transfected with a cDNA encoding the bovine CD3epsilon chain, reacted with mAb MM1A indicating that the epitope recognised is on the epsilon chain of the complex and confirming that the mAb recognised bovine CD3.
我们描述了一种单克隆抗体（mAb）MM1A的特性，它能与牛αβ和γδTcR⁺ T细胞表面的一种抗原分子发生反应。该单克隆抗体免疫沉淀出一系列分子量分别为21 kDa、22 kDa、32 kDa、36 kDa和44 kDa的多肽，这与其识别TcR/CD3复合物相符。用编码牛CD3ε链的cDNA转染的COS细胞与单克隆抗体MM1A发生反应，表明所识别的表位位于该复合物的ε链上，并证实该单克隆抗体识别牛CD3。
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