Reynolds L P, Redmer D A
Department of Animal and Range Sciences, North Dakota State University, Fargo 58105.
Biol Reprod. 1992 Nov;47(5):698-708. doi: 10.1095/biolreprod47.5.698.
Growth and microvascular development of the uterus were evaluated for ewes on Days 12, 18, 24, and 30 after mating (3-4 ewes/day; Day 0 = day of mating) in two experiments. In experiment 1, fresh weight and dry weight of gravid uterine horns were increased on Days 24 and 30 after mating, whereas those of nongravid uterine horns were elevated only on Day 30. The increased fresh and dry weights of gravid uterine horns on Day 24 were associated with uterine hyperplasia (increased DNA content). Increased fresh and dry weights of gravid uterine horns on Day 30, however, were associated with hypertrophy (increased RNA:DNA and protein:DNA ratios) of uterine tissues. In experiment 2, vascularity of endometrial tissues was elevated on Days 24 and 30 after mating. In addition, dramatic changes in uterine architecture (increased lumenal diameter and decreased endometrial thickness) and in uterine microvascular development (increased abundance of large microvessels and development of a subepithelial capillary plexus) were observed by Day 24 after mating. Characterization of the patterns of uterine growth and microvascular development will enable us to further define the role of previously reported uterine and conceptus-derived growth and angiogenic factors during early pregnancy.
在两项实验中，对配种后第12、18、24和30天的母羊子宫生长和微血管发育进行了评估（每天3 - 4只母羊；第0天 = 配种日）。在实验1中，妊娠子宫角的鲜重和干重在配种后第24天和第30天增加，而非妊娠子宫角仅在第30天增加。妊娠子宫角在第24天鲜重和干重的增加与子宫增生（DNA含量增加）有关。然而，妊娠子宫角在第30天鲜重和干重的增加与子宫组织肥大（RNA:DNA和蛋白质:DNA比率增加）有关。在实验2中，子宫内膜组织的血管形成在配种后第24天和第30天增加。此外，在配种后第24天观察到子宫结构发生显著变化（管腔直径增加和子宫内膜厚度减小）以及子宫微血管发育发生显著变化（大型微血管数量增加和上皮下毛细血管丛形成）。对子宫生长和微血管发育模式的表征将使我们能够进一步确定先前报道的子宫和胚胎来源的生长及血管生成因子在早期妊娠中的作用。
