Reynolds L P, Kirsch J D, Kraft K C, Knutson D L, McClaflin W J, Redmer D A
Department of Animal & Range Sciences, North Dakota State University, Fargo, 58105, USA.
Biol Reprod. 1998 Sep;59(3):606-12. doi: 10.1095/biolreprod59.3.606.
The time-course of uterine growth, cell proliferation, and microvascular development was evaluated during the first 72 h after implanting estradiol-17beta (E2) into ovariectomized (OVX) ewes. Uterine fresh weight increased 2.3-fold by 24 h and increased further (3.3-fold) by 48 h. The majority (approximately 75%) of this growth response was associated with tissue growth rather than a change in the tissue dry weight:fresh weight ratio. Both uterine cell number (DNA content) and cell size (RNA:DNA ratio) increased from 0 to 24 h (1.8-fold and 1.7-fold, respectively). Cell proliferation also increased dramatically between 8 h and 24 h after E2 implantation. Endometrial microvascular volume density (percentage of tissue volume occupied by microvessels) increased approximately 1.8-fold by 24 h and then remained constant or declined slightly through 72 h. The total endometrial microvascular volume, however, increased approximately 5-fold from 0 to 24 h and increased further by 72 h. Thus, treatment of OVX ewes with E2 caused a dramatic increase in uterine fresh and dry weights by 24 h, due primarily to hyperplasia and hypertrophy, with only a relatively small change in tissue dry weight:fresh weight ratio. This dramatic uterine growth was associated with a profound increase in endometrial microvascular volume.
在给去卵巢（OVX）母羊植入17β-雌二醇（E2）后的最初72小时内，评估了子宫生长、细胞增殖和微血管发育的时间进程。子宫鲜重在24小时时增加了2.3倍，在48小时时进一步增加（3.3倍）。这种生长反应的大部分（约75%）与组织生长有关，而不是组织干重与鲜重之比的变化。子宫细胞数量（DNA含量）和细胞大小（RNA:DNA比值）在0至24小时之间均增加（分别为1.8倍和1.7倍）。在E2植入后8至24小时之间，细胞增殖也显著增加。子宫内膜微血管体积密度（微血管占据的组织体积百分比）在24小时时增加了约1.8倍，然后在72小时内保持不变或略有下降。然而，子宫内膜微血管总体积在0至24小时之间增加了约5倍，并在72小时时进一步增加。因此，用E2处理OVX母羊在24小时时导致子宫鲜重和干重显著增加，主要是由于细胞增生和肥大，而组织干重与鲜重之比仅有相对较小的变化。这种显著的子宫生长与子宫内膜微血管体积的大幅增加有关。
