Departments of Animal Sciences, North Dakota State University, Fargo, ND.
Department of Animal and Range Sciences, Montana State University, Bozeman, MT.
J Anim Sci. 2018 Jul 28;96(8):3471-3481. doi: 10.1093/jas/sky221.
The hypothesis of this study was that arginine supplementation would overcome negative effects of restricted maternal feed intake during the last two-thirds of gestation on ewe performance and positively affect postnatal lamb growth and development. Multiparous, Rambouillet ewes (n = 32) were allocated to 3 treatments in a completely random design at 54 ± 3.9 d of gestation. Dietary treatments were 100% of nutrient requirements (control, CON), 60% of control (restricted, RES), or RES plus a rumen-protected arginine supplement dosed at 180 mg/kg BW once daily (RES-ARG). Ewes were penned individually in a temperature-controlled facility. At parturition, lambs were immediately removed from dams and reared independently. At day 54 ± 3 of age, lambs were stunned using captive bolt, exsanguinated, and organs were collected and weighed. Ewe BW from day 68 of gestation through parturition was greater (P ≤ 0.03) in CON compared with RES or RES-ARG. Similarly, ewe BCS from day 68 of gestation through parturition was greater (P ≤ 0.03) in CON than either RES or RES-ARG. Total ewe colostrum mass (g) at 3 h after parturition was greater (P ≤ 0.001) in CON than RES or RES-ARG. Lamb birth weight was greater (P = 0.04) in CON than RES ewes and tended (P = 0.10) to be greater in CON vs. RES-ARG. Lambs born to CON ewes had greater (P ≤ 0.03) BW than lambs from RES ewes at 7, 14, and 33 d postpartum. On day 19, lambs from CON and RES-ARG ewes both had greater (P ≤ 0.04) BW than lambs from RES ewes (12.0 and 11.5 vs. 10.3 ± 0.41 kg, respectively). Lambs born to CON and RES-ARG ewes had greater (P ≤ 0.04) ADG than lambs from RES ewes on day 19 (355.0 and 354.0 vs. 306.4 ± 15.77 g, respectively). Lambs from CON and RES-ARG ewes also had greater (P ≤ 0.02) girth circumference than lambs from RES ewes on day 19 (55.4 and 54.6 vs. 51.3 ± 0.97 cm, respectively). On day 54, lambs from RES-ARG ewes had greater (P = 0.003) curved crown rump length than lambs from RES ewes (99.8 vs. 93.9 ± 1.28 cm, respectively). Adrenal glands in lambs from CON dams had greater (P = 0.01) mass than adrenal glands in lambs from RES dams. Livers from lambs born to RES-ARG ewes weighed more (P = 0.05) than livers from lambs born to RES ewes. These results confirm our hypothesis that arginine supplementation during the last two-thirds of gestation can mitigate offspring, but not maternal negative consequences associated with restricted maternal nutrition.
本研究的假设是，精氨酸补充剂将克服妊娠最后三分之二期间限制母羊采食量对母羊性能的负面影响，并积极影响产后羔羊的生长和发育。在妊娠 54 ± 3.9 天时，将 32 只多产的拉曼特母羊按完全随机设计分配到 3 个处理中。日粮处理分别为 100%营养需求（对照，CON）、60%对照（限制，RES）或 RES 加每日 180 毫克/公斤 BW 的包膜精氨酸补充剂（RES-ARG）。母羊单独在温度可控的设施中饲养。分娩时，羔羊立即从母羊身上取出并独立饲养。在 54 ± 3 日龄时，羔羊使用固定头撞昏，放血，收集和称重器官。妊娠第 68 天至分娩时，CON 组母羊 BW 高于 RES 或 RES-ARG（P≤0.03）。同样，妊娠第 68 天至分娩时，CON 组母羊 BCS 高于 RES 或 RES-ARG（P≤0.03）。分娩后 3 小时，CON 组母羊总初乳量（克）高于 RES 或 RES-ARG（P≤0.001）。羔羊出生体重在 CON 组高于 RES 母羊（P=0.04），且在 CON 组与 RES-ARG 组之间有趋势（P=0.10）高于 RES 母羊。CON 组羔羊在产后 7、14 和 33 天时 BW 均高于 RES 组羔羊（分别为 12.0 和 11.5 与 10.3±0.41kg）。第 19 天，CON 和 RES-ARG 组羔羊 BW 均高于 RES 组羔羊（分别为 12.0 和 11.5 与 10.3±0.41kg）（P≤0.04）。第 19 天，CON 和 RES-ARG 组羔羊 ADG 高于 RES 组羔羊（分别为 355.0 和 354.0 与 306.4±15.77g）（P≤0.04）。第 19 天，CON 和 RES-ARG 组羔羊胸围也大于 RES 组羔羊（分别为 55.4 和 54.6 与 51.3±0.97cm）（P≤0.02）。第 54 天，RES-ARG 组羔羊的曲冠状嵴长（CCL）大于 RES 组羔羊（99.8 与 93.9±1.28cm）（P=0.003）。CON 组母羊的肾上腺质量大于 RES 组母羊（P=0.01）。RES-ARG 组羔羊的肝脏重量大于 RES 组羔羊（P=0.05）。这些结果证实了我们的假设，即在妊娠的最后三分之二期间补充精氨酸可以减轻与限制母羊营养相关的后代的负面影响，但不能减轻母羊的负面影响。
