Guo J T, Chen H S, Tang X S
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Beijing.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 1992 Aug;12(8):480-2, 454.
The method of DHBV replication complexes (RCs) purification was modified. In order to screen anti-HBV drugs from Chinese medicinal herbs, the inhibitory effects of the extracts of 14 Chinese recipes and herbs, 30 compounds isolated from Chinese herbs on DHBV DNA polymerase (DNAP) and reverse transcriptase (RT) have been studied. The results showed that extracts of Xiao Chai Hu Tang (small Bupleurum decoction) inhibited DHBV DNAP and RT in less extent. Of the 7 herbs, the components of Xiao Chai Hu Tang, the extracts of S. baicalensis and P. ternata potently inhibited DHBV RT, their concentration of reducing enzyme activity by 50% (IC50) was 1.25 and 1.6 mg/ml respectively. Furthermore, it has been proved that S. baicalensis inhibited DHBV DNA replication in ducklings. It also was found the extract of P. cuspidatum inhibited DHBV RT with IC50 of 1.76 mg/ml. Nine of thirty isolated compounds inhibited both DHBV DNAP and RT in less extent under high concentration, while other did not.
对鸭乙肝病毒（DHBV）复制复合物（RCs）的纯化方法进行了改进。为了从中药中筛选抗乙肝病毒药物，研究了14种中药方剂和草药提取物、从中草药中分离得到的30种化合物对DHBV DNA聚合酶（DNAP）和逆转录酶（RT）的抑制作用。结果表明，小柴胡汤提取物对DHBV DNAP和RT的抑制作用较小。在这7种草药中，小柴胡汤的成分、黄芩和半夏提取物对DHBV RT有较强的抑制作用，其使酶活性降低50%的浓度（IC50）分别为1.25和1.6mg/ml。此外，已证实黄芩可抑制雏鸭体内的DHBV DNA复制。还发现虎杖提取物对DHBV RT有抑制作用，IC50为1.76mg/ml。30种分离化合物中有9种在高浓度下对DHBV DNAP和RT的抑制作用较小，而其他化合物则无此作用。
