Tonks A J, Cooper R A, Jones K P, Blair S, Parton J, Tonks A
Department of Haematology, University of Wales College of Medicine, Heath Park, Cardiff, Wales CF14 4XN, UK.
Cytokine. 2003 Mar 7;21(5):242-7. doi: 10.1016/s1043-4666(03)00092-9.
Clinical observations indicate that honey may initiate or accelerate the healing of chronic wounds and has, therefore, been claimed to have anti-inflammatory properties. The aim of this study was to investigate the effects of honey on the activation state of immunocompetent cells, using the monocytic cell line, MonoMac-6 (MM6), as a model. We investigated the effect of each of the three honeys (manuka, pasture and jelly bush) on the release of important inflammatory cytokines from MM6 cells. These honeys, together with a sugar syrup control (artificial honey), were incubated with MM6 cells at a concentration of 1% (w/v) for 0-24h. Cell culture supernatants were tested using specific ELISA assays for tumour necrosis factor-alpha (TNF-alpha) and interleukin (IL)-1beta and IL-6. All honeys significantly increased the TNF-alpha, IL-1beta and IL-6 release from MM6 cells (and human monocytes) when compared with untreated and artificial-honey-treated cells (P<0.001). Jelly bush honey significantly induced the maximal release of each cytokine compared with manuka, pasture or artificial honeys (P<0.001). These results suggest that the effect of honey on wound healing may in part be related to the stimulation of inflammatory cytokines from monocytic cells. Such cell types are known to play an important role in healing and tissue repair.
临床观察表明，蜂蜜可能启动或加速慢性伤口的愈合，因此，有人声称它具有抗炎特性。本研究的目的是使用单核细胞系MonoMac-6（MM6）作为模型，研究蜂蜜对免疫活性细胞激活状态的影响。我们研究了三种蜂蜜（麦卢卡蜂蜜、牧草蜂蜜和果冻树蜂蜜）中的每一种对MM6细胞释放重要炎性细胞因子的影响。将这些蜂蜜与糖浆对照（人工蜂蜜）以1%（w/v）的浓度与MM6细胞一起孵育0 - 24小时。使用特异性ELISA测定法检测细胞培养上清液中的肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β和IL-6。与未处理和人工蜂蜜处理的细胞相比，所有蜂蜜均显著增加了MM6细胞（和人单核细胞）中TNF-α、IL-1β和IL-6的释放（P<0.001）。与麦卢卡蜂蜜、牧草蜂蜜或人工蜂蜜相比，果冻树蜂蜜显著诱导了每种细胞因子的最大释放（P<0.001）。这些结果表明，蜂蜜对伤口愈合的作用可能部分与刺激单核细胞产生炎性细胞因子有关。已知这类细胞在愈合和组织修复中起重要作用。
