Bertin Nadia, Genard Michel, Fishman Svetlana
Unité Plantes et Systèmes de culture Horticoles, INRA, Site Agroparc, F-84914 Avignon Cedex 9, France.
Ann Bot. 2003 Jul;92(1):65-72. doi: 10.1093/aob/mcg111.
Changes in cell number during the early period of tomato fruit development were analysed by means of a deterministic model of cell multiplication. The period commenced at the seed stage with one theoretical cell undergoing intensive cell division, and ended when the cell number became nearly constant. The model takes into consideration the proliferative activity of the fruit cell population which, a few days before flower anthesis, begins to decrease progressively after each mitotic cycle. Model parameters, namely the time at which proliferative activity diminishes, its rate of decrease and the length of the cell cycle, were estimated by fitting the model to observed cell population dynamics in tomato fruits growing in three different positions on the truss. It is hypothesized that the molecular mechanism responsible for the cessation of mitosis in growing fruits is associated with shortening of telomeric ends of nuclear DNA, as suggested previously for other growing cell populations.
通过细胞增殖的确定性模型分析了番茄果实发育早期的细胞数量变化。这个时期始于种子期，当时一个理论细胞开始进行密集的细胞分裂，结束于细胞数量几乎保持恒定之时。该模型考虑了果实细胞群体的增殖活性，在花开放前几天，这种活性在每个有丝分裂周期后开始逐渐下降。通过将模型与在果穗上三个不同位置生长的番茄果实中观察到的细胞群体动态进行拟合，估计了模型参数，即增殖活性降低的时间、其降低速率以及细胞周期的长度。据推测，正如之前对其他生长细胞群体所表明的那样，负责生长果实中有丝分裂停止的分子机制与核DNA端粒末端的缩短有关。
