Pandey Ajay K, Reddy M Sudhakara, Suryanarayanan Trichur S
Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala 147 001, India.
Mycol Res. 2003 Apr;107(Pt 4):439-44. doi: 10.1017/s0953756203007494.
Different isolates of a foliar endophytic species of Phyllosticta were isolated from different tropical tree species in India to examine genetic variation among the isolates. Internal transcribed spacer-restriction fragment length polymorphism (ITS-RFLP) analysis did not detect any variation among the isolates, suggesting that they all belong to the same species. Sequence data of the ITS region of ribosomal DNA (ITS1 and ITS2, including 5.8S rDNA) supported the identity of the present fungal isolates as P. capitalensis. These results show that P. capitalensis (teleomorph Guignardia endophyllicola?) is an ubiquitous foliar endophyte that can infect tree hosts from different families and habitats.
从印度不同的热带树种中分离出叶内生叶点霉属的不同分离株，以检测这些分离株之间的遗传变异。内转录间隔区-限制性片段长度多态性（ITS-RFLP）分析未检测到分离株之间的任何变异，表明它们都属于同一物种。核糖体DNA的ITS区域（ITS1和ITS2，包括5.8S rDNA）的序列数据支持了当前真菌分离株为卡氏叶点霉的鉴定。这些结果表明，卡氏叶点霉（有性型为内生叶基盘菌？）是一种广泛存在的叶内生菌，可感染来自不同科和生境的树木宿主。
