Kim Mun-Ok, Kim Gi-Young, Nam Byung-Hyouk, Jin Cheng-Yun, Lee Ki-Won, Park Jae-Min, Lee Sang-Joon, Lee Jae-Dong
Department of Microbiology, College of Natural Sciences, Pusan National University, Pusan 609-735, Korea.
Mycobiology. 2005 Jun;33(2):104-8. doi: 10.4489/MYCO.2005.33.2.104. Epub 2005 Jun 30.
Genus Phellinus taxonomically belongs to Aphyllophorales and some species of this genus have been used as a medicinal ingredients and Indian folk medicines. Especially, P. linteus and morphological-related species are well-known medicinal fungi that have various biological activities such as humoral and cell-mediated, anti-mutagenic, and anti-cancer activities. However, little is known about the rapid detection for complex Phellinus species. Therefore, this study was carried out to develop specific primers for the rapid detection of P. linteus and other related species. Designing the species-specific primers was done based on internal transcribed spacer sequence data. Each primer set detected specifically P. linteus (PL2/PL5R) and P. baumii (PB1/PB4R). These primer sets could be useful for the rapid detection of specific-species among unidentified Phellinus species. Moreover, restriction fragment length polymorphism analysis of the ITS region with HaeIII was also useful for clarifying the relationship between each 5 Phellinus species.
桑黄属在分类学上属于非褶菌目，该属的一些物种已被用作药用成分和印度民间药物。特别是，裂蹄木层孔菌及其形态相关物种是著名的药用真菌，具有多种生物活性，如体液免疫和细胞介导免疫、抗诱变和抗癌活性。然而，对于复杂的桑黄属物种的快速检测知之甚少。因此，本研究旨在开发用于快速检测裂蹄木层孔菌和其他相关物种的特异性引物。基于内转录间隔区序列数据设计物种特异性引物。每个引物组可特异性检测裂蹄木层孔菌（PL2/PL5R）和鲍氏木层孔菌（PB1/PB4R）。这些引物组可用于快速检测未鉴定的桑黄属物种中的特定物种。此外，用HaeIII对ITS区域进行限制性片段长度多态性分析也有助于阐明5种桑黄属物种之间的关系。
