Castro Bruno B, Guilhermino Lúcia, Ribeiro Rui
Instituto do Ambiente e Vida, Departamento de Zoologia da Universidade de Coimbra, Largo Marquês de Pombal, 3004-517 Coimbra, Portugal.
Environ Pollut. 2003;125(3):325-35. doi: 10.1016/s0269-7491(03)00120-9.
The purpose of this study was to develop an in situ sediment bioassay chamber and respective procedures, suitable for performing toxicity bioassays with benthic invertebrates, using the midge Chironomus riparius. It was also our objective to compare the responses obtained under controlled conditions (laboratory 10-day larval growth and survival test) with those obtained in situ. Clean sand and a formulated sediment were incorporated in the in situ bioassay, along with local sediments, as a way of minimizing natural variability due to physicochemical differences among sediments or due to interactions with indigenous organisms. Recovery of organisms was good (80-100% in the control and reference site), indicating that the developed chamber and protocol were suitable for exposing and retrieving C. riparius in situ. Results also showed differences between responses obtained with formulated and natural sediment in situ, as well as between laboratory and in situ.
本研究的目的是开发一种原位沉积物生物测定室及相应程序，该程序适用于使用摇蚊（Chironomus riparius）对底栖无脊椎动物进行毒性生物测定。我们的目标还包括将在受控条件下（实验室10天幼虫生长和存活试验）获得的反应与原位获得的反应进行比较。原位生物测定中加入了清洁沙子、配制沉积物以及当地沉积物，以此尽量减少由于沉积物间物理化学差异或与本地生物相互作用导致的自然变异性。生物恢复情况良好（对照和参考地点为80 - 100%），这表明所开发的生物测定室和方案适用于原位暴露和回收溪流摇蚊。结果还显示了原位使用配制沉积物和天然沉积物获得的反应之间以及实验室和原位之间的差异。
