Oller-López J L, Avalos J, Barrero A F, Oltra J E
Departamento de Química Orgánica, Instituto de Biotecnología, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain.
Appl Microbiol Biotechnol. 2003 Dec;63(3):282-5. doi: 10.1007/s00253-003-1376-6. Epub 2003 Jun 26.
Recent studies into gibberellin A(1) (GA(1)) showed it to be physiologically more active than GA(3) in plants of great agricultural interest, such as tomatoes, rice, peas, and sweet cherries. We describe here a simple procedure for obtaining large quantities of GA(1) (1,500 mg/l) by incubating the FKMC1995 strain of Fusarium fujikuroi in a standard complex medium (SCM). We also compare the GA production of this strain with that of two other wild-type strains of F. fujikuroi (IMI58289, m567) in SCM and low-nitrogen medium and discuss the possible biogenetic mechanisms involved in the over-accumulation of GA(1) by FKMC1995.
最近对赤霉素A(1)(GA(1))的研究表明,在番茄、水稻、豌豆和甜樱桃等具有重大农业价值的植物中,GA(1)在生理上比GA(3)更具活性。我们在此描述了一种简单的方法,通过在标准复合培养基(SCM)中培养藤仓镰孢菌的FKMC1995菌株来大量获得GA(1)(1500毫克/升)。我们还比较了该菌株与藤仓镰孢菌的另外两个野生型菌株(IMI58289,m567)在SCM和低氮培养基中的GA产量,并讨论了FKMC1995菌株中GA(1)过度积累可能涉及的生物合成机制。