Laboratorio de Bioorgánica, Departamento de Química, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile.
Phytochemistry. 2010 Aug;71(11-12):1322-31. doi: 10.1016/j.phytochem.2010.05.006.
Several isolates of three Fusarium species associated with the Gibberella fujikuroi species complex were characterized for their ability to synthesize gibberellins (GAs): Fusarium sacchari (mating population B), Fusarium konzum (mating population I) and Fusarium subglutinans (mating population E). Of these, F. sacchari is phylogenetically related to Fusarium fujikuroi and is grouped in the Asian clade of the complex, while F. konzum and F. subglutinans are only distantly related to Fusarium fujikuroi and belong to the American clade. Variability was found between the different F. sacchari strains tested. Five isolates (B-12756; B-1732, B-7610, B-1721 and B-1797) were active in GA biosynthesis and accumulated GA(3) in the culture fluid (2.76-28.4 microg/mL), while two others (B-3828 and B-1725) were inactive. GA(3) levels in strain B-12756 increased by 2.9 times upon complementation with ggs2 and cps-ks genes from F. fujikuroi. Of six F. konzum isolates tested, three (I-10653; I-11616; I-11893) synthesized GAs, mainly GA(1), at a low level (less than 0.1 microg/mL). Non-producing F. konzum strains contained no GA oxidase activities as found for the two F. subglutinans strains tested. These results indicate that the ability to produce GAs is present in other species of the G. fujikuroi complex beside F. fujikuroi, but might differ significantly in different isolates of the same species.
几种与藤仓赤霉种复合体相关的镰刀菌属真菌的分离株，其合成赤霉素（GA）的能力如下：甘蔗镰刀菌（交配群体 B）、尖孢镰刀菌（交配群体 I）和亚粘帚霉（交配群体 E）。其中，甘蔗镰刀菌在系统发育上与藤仓赤霉有关，并且被归类于该复合体的亚洲分支，而尖孢镰刀菌和亚粘帚霉与藤仓赤霉的亲缘关系较远，属于美国分支。在所测试的不同甘蔗镰刀菌菌株之间发现了变异性。五个分离株（B-12756；B-1732、B-7610、B-1721 和 B-1797）在 GA 生物合成中具有活性，并在培养液中积累 GA(3)（2.76-28.4μg/mL），而另外两个（B-3828 和 B-1725）则无活性。当用藤仓赤霉的 ggs2 和 cps-ks 基因进行互补时，菌株 B-12756 中的 GA(3)水平增加了 2.9 倍。在所测试的六个尖孢镰刀菌分离株中，有三个（I-10653；I-11616；I-11893）以低水平（低于 0.1μg/mL）合成 GA，主要是 GA(1)。非产 GA 的尖孢镰刀菌菌株中未发现 GA 氧化酶活性，与所测试的两个亚粘帚霉菌株相同。这些结果表明，除了藤仓赤霉之外，藤仓赤霉复合体中的其他种也具有产生 GA 的能力，但在同一物种的不同分离株中可能存在显著差异。
