[通过在不同载体上进行凝胶过滤和高压液相色谱法研究免疫球蛋白制剂的组分比例]

[The fractional composition of immunoglobulin preparations studied by gel filtration on different carriers and by high-pressure liquid chromatography].

作者信息

Kargina T M, Runova V F, Migunov V N, Zeĭnalov A M, Azhigirova M A, Rusanov V M, Pozina I M, Legat E M

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1992(9-10):48-50.

Abstract

The fractional composition of immunoglobulin preparations produced by different manufacturing enterprises of this country has been studied by gel chromatography in columns packed with different carriers (Sephadex G-200 and ultragel AcA-34) and by high-performance liquid chromatography (HPLC). This study has revealed the nonstandard character of immunoglobulin preparations produced according to the same technological procedure (modified Cohn's method). The fractionation of immunoglobulins on different carriers with the use of different methods has yielded similar results confirmed by the statistical processing of the data. The results obtained in the study of the fractional composition of immunoglobulin preparations evidence that gel filtration with the use of ultragel and HPLC have greater resolving capacity in comparison with the method of gel filtration on traditionally used Sephadex G-200.

摘要

该国不同生产企业生产的免疫球蛋白制剂的组分，已通过填充不同载体（葡聚糖凝胶G - 200和超凝胶AcA - 34）的柱凝胶色谱法以及高效液相色谱法（HPLC）进行了研究。该研究揭示了按照相同工艺流程（改良的科恩方法）生产的免疫球蛋白制剂具有不标准的特性。使用不同方法在不同载体上对免疫球蛋白进行分级分离，得到了相似的结果，这些结果经数据统计处理得到证实。对免疫球蛋白制剂组分研究所得结果表明，与传统使用的葡聚糖凝胶G - 200凝胶过滤法相比，使用超凝胶的凝胶过滤法和HPLC具有更强的分辨能力。