Tamura K, Manabe T, Imanishi K, Nishikawa H, Ohshio G, Tobe T
First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Hepatogastroenterology. 1992 Dec;39(6):536-9.
Isolated pancreatic acini were incubated with either a combination of xanthine and xanthine oxidase which generates superoxide (O2), or hydrogen peroxide (H2O2), and the direct cytotoxic effect of active oxygen species on the pancreatic acini was examined in vitro in the isolated pancreatic acini system of the rat. Both amylase secretion and lactic dehydrogenase discharge were increased dose-dependently by the addition of xanthine and xanthine oxidase, and suppressed by the addition of a superoxide scavenger, superoxide dismutase. In addition, amylase and lectate dehydrogenase discharge was increased dose-dependently by hydrogen peroxide and decreased by catalase. These results suggest that superoxide and hydrogen peroxide directly injure pancreatic acinar cells and that active oxygen species are involved in the pathogenesis of acute pancreatitis.
将分离的胰腺腺泡与能产生超氧阴离子（O₂）的黄嘌呤和黄嘌呤氧化酶组合，或过氧化氢（H₂O₂）一起孵育，并在大鼠分离的胰腺腺泡系统中体外检测活性氧对胰腺腺泡的直接细胞毒性作用。添加黄嘌呤和黄嘌呤氧化酶后，淀粉酶分泌和乳酸脱氢酶释放均呈剂量依赖性增加，而添加超氧阴离子清除剂超氧化物歧化酶后则受到抑制。此外，过氧化氢可使淀粉酶和乳酸脱氢酶释放呈剂量依赖性增加，而过氧化氢酶则可使其降低。这些结果表明，超氧阴离子和过氧化氢可直接损伤胰腺腺泡细胞，活性氧参与了急性胰腺炎的发病机制。
