Platt Ratree, Reynolds Donald L, Phillips Gregory J
Department of Veterinary Microbiology and Preventive Medicine, 1802 Elwood Drive, Veterinary Medical Research Institute, Building # 6, Iowa State University, Ames 50011, USA.
FEMS Microbiol Lett. 2003 Jun 27;223(2):259-65. doi: 10.1016/S0378-1097(03)00388-4.
Bacteriophage therapy represents a potential alternative to the use of antibiotics to control proliferation of pathogenic bacteria. As an alternative to the strategy where a limited number of doses of large numbers of lytic bacteriophages are administered, a novel method delivery system was developed so that phages are continually released into the culture. Specifically, a non-pathogenic Escherichia coli strain was constructed that was lysogenic for a lytic mutant of bacteriophage lambda. This lysogen was shown to be effective at decreasing the number of lambda-sensitive E. coli in vitro. Construction of this E. coli strain was accomplished by development of a plasmid-based system utilizing the site-specific recombination machinery of bacteriophage P22 to integrate DNA constructs into the host chromosome. This recombination system is useful for strain construction and other genetic manipulations in both E. coli and Salmonella enterica serovars.
噬菌体疗法是一种控制病原菌增殖的潜在抗生素替代方法。作为大量裂解性噬菌体有限剂量给药策略的替代方法，开发了一种新型递送系统，使噬菌体持续释放到培养物中。具体而言，构建了一种非致病性大肠杆菌菌株，它对噬菌体λ的裂解突变体具有溶原性。该溶原菌在体外能有效减少对λ敏感的大肠杆菌数量。这种大肠杆菌菌株的构建是通过开发一种基于质粒的系统来完成的，该系统利用噬菌体P22的位点特异性重组机制将DNA构建体整合到宿主染色体中。这种重组系统对于大肠杆菌和肠炎沙门氏菌血清型的菌株构建及其他基因操作很有用。
