噬菌体λ和P22编码的功能对大肠杆菌中recF依赖性重组的激活作用。
Activation of recF-dependent recombination in Escherichia coli by bacteriophage lambda- and P22-encoded functions.
作者信息
Poteete A R, Volkert M R
机构信息
Department of Molecular Genetics and Microbiology, University of Massachusetts, Worcester 01655.
出版信息
J Bacteriol. 1988 Sep;170(9):4379-81. doi: 10.1128/jb.170.9.4379-4381.1988.
Abstract
Escherichia coli strains bearing wild-type and mutant alleles of various recombination genes, as well as plasmids that express recombination-related genes of bacteriophages lambda and P22, were tested for their proficiency as recipients in Hfr-mediated conjugation. It was found that the homologous recombination systems of both phages could promote recombination in a recB recC mutant host. In addition, the Abc function of P22, but not the Gam function of lambda, was found to inhibit recombination in a wild-type host; however, both Abc and Gam inhibited recombination in a recF mutant host. These observations are interpreted as indicating that the recombination systems of both phages, as well as the RecBCD-modulating functions Abc and Gam, all activate the RecF recombination pathway of E. coli.
摘要
对携带各种重组基因野生型和突变等位基因的大肠杆菌菌株，以及表达噬菌体λ和P22重组相关基因的质粒，进行了Hfr介导的接合中作为受体菌的能力测试。结果发现，两种噬菌体的同源重组系统均可在recB recC突变宿主中促进重组。此外，发现P22的Abc功能而非λ的Gam功能可在野生型宿主中抑制重组；然而，Abc和Gam在recF突变宿主中均抑制重组。这些观察结果被解释为表明两种噬菌体的重组系统以及RecBCD调节功能Abc和Gam均激活了大肠杆菌的RecF重组途径。
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本文引用的文献
1
Acetylornithinase of Escherichia coli: partial purification and some properties.
J Biol Chem. 1956 Jan;218(1):97-106.
2
Genetic analysis of the erf region of the bacteriophage P22 chromosome.
Virology. 1984 Apr 15;134(1):148-60. doi: 10.1016/0042-6822(84)90280-0.
3
Role of the recF gene of Escherichia coli K-12 in lambda recombination.
Mol Gen Genet. 1981;181(4):497-504. doi: 10.1007/BF00428742.
4
Genetic analysis of the RecE pathway of genetic recombination in Escherichia coli K-12.
J Bacteriol. 1981 Jan;145(1):521-32. doi: 10.1128/jb.145.1.521-532.1981.
5
RecF and RecBC function during recombination of nonreplicating, UV-irradiated phage lambda DNA and during other recombination processes.
Cold Spring Harb Symp Quant Biol. 1984;49:475-83. doi: 10.1101/sqb.1984.049.01.054.
6
Lambda red-dependent growth and recombination of phage P22.
Virology. 1984 Apr 15;134(1):161-7. doi: 10.1016/0042-6822(84)90281-2.
7
Expression of the phage lambda recombination genes exo and bet under lacPO control on a multi-copy plasmid.
Gene. 1983 Sep;23(3):277-92. doi: 10.1016/0378-1119(83)90018-5.
8
Recombination in bacteriophage lambda. I. Mutants deficient in general recombination.
J Mol Biol. 1968 Jul 14;34(2):261-71. doi: 10.1016/0022-2836(68)90251-9.
9
Mutants of bacteriophage lambda defective in vegetative genetic recombination.
J Mol Biol. 1968 Jul 14;34(2):239-49. doi: 10.1016/0022-2836(68)90249-0.
10
A recombination function essential to the growth of bacteriophage P22.
J Mol Biol. 1970 Dec 28;54(3):417-40. doi: 10.1016/0022-2836(70)90119-1.
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