Bock Troels, Pakkenberg Bente, Buschard Karsten
H:S Bartholin Institute, Copenhagen University Hospital, Copenhagen, Denmark.
Diabetes. 2003 Jul;52(7):1716-22. doi: 10.2337/diabetes.52.7.1716.
It is important for our understanding of the pancreatic islets to study whether new islets are able to form in the intact pancreas. We developed a new method to determine the total number and the mean volume of the pancreatic islets, and we used this method to study the expansion of the islet mass in ob/ob mice (n = 8), using ob/+ mice (n = 8) as controls. The total islet volume was increased by a factor of 3.6 in ob/ob mice compared with ob/+ mice, whereas, importantly, the total number of islets did not differ among ob/ob mice and ob/+ mice (3,193 +/- 160 islets in ob/ob mice vs. 3,184 +/- 142 islets in ob/+ mice, P = 0.97). The coefficient of variation in the volume distribution of islets was equal in the two groups, showing that in ob/ob mice, the existing islets expand their volume by the same proportion, without a net formation of new islets. We suggest that the pancreatic islets should be considered as anatomically such complex structures that islet neogenesis does not spontaneously occur in an intact pancreas. Cells within the existing islets are presumably the most important sources for islet cell hyperplasia during expansion of the total islet mass.
研究完整胰腺中是否能够形成新的胰岛，对于我们理解胰岛至关重要。我们开发了一种新方法来确定胰岛的总数和平均体积，并使用该方法研究ob/ob小鼠（n = 8）胰岛质量的增长情况，以ob/+小鼠（n = 8）作为对照。与ob/+小鼠相比，ob/ob小鼠的胰岛总体积增加了3.6倍，而重要的是，ob/ob小鼠和ob/+小鼠之间的胰岛总数没有差异（ob/ob小鼠为3,193 ± 160个胰岛，ob/+小鼠为3,184 ± 142个胰岛，P = 0.97）。两组胰岛体积分布的变异系数相等，表明在ob/ob小鼠中，现有的胰岛以相同比例扩大其体积，没有新胰岛的净形成。我们认为胰岛在解剖学上应被视为如此复杂的结构，以至于在完整胰腺中不会自发发生胰岛新生。在胰岛总体积扩大过程中，现有胰岛内的细胞可能是胰岛细胞增生的最重要来源。
