Uchida K, Fujiwara H
Yakult Central Institute for Microbiological Research, Tokyo, Japan.
Jikken Dobutsu. 1988 Oct;37(4):421-8. doi: 10.1538/expanim1978.37.4_421.
Male Small-obese mice (Small-ob) which derived from a C57BL/6 J-ob/ob mouse colony were examined histopathologically at 13-, 39-, and over 52-week-old. C57BL/6 J-ob mice (?/+: Non-ob, ob/ob: Ob) were studied as controls. In Small-ob mice, plasma glucagon concentration was higher than that of the Ob mice (this difference was highly significant), and serum levels for insulin was within normal limits. Microscopically, hypertrophy and hyperplasia of the islets of Langerhans were found only in the pancreas of Ob mice. The increase in the number of A-cells and the decrease in the number of B-cells were revealed immunohistochemically in the islets of Small-ob mice. These changes were more severe with advance of age. In the aged Small-ob mice, perivascular and periductular cell infiltration were found, but inflammatory change of islet tissue was not confirmed in any animals examined. Diabetic symptoms in Small-ob mice seems to stem from the disparity in insulin/glucagon (I/G) ratio associated with hyperglucagonemia which result from increased number of A-cells of pancreatic islets.
对源自C57BL/6 J-ob/ob小鼠群体的雄性小肥胖小鼠（Small-ob）在13周龄、39周龄和52周龄以上时进行了组织病理学检查。将C57BL/6 J-ob小鼠（？/+：非肥胖，ob/ob：肥胖）作为对照进行研究。在Small-ob小鼠中，血浆胰高血糖素浓度高于肥胖小鼠（这种差异非常显著），而胰岛素的血清水平在正常范围内。显微镜下，仅在肥胖小鼠的胰腺中发现胰岛肥大和增生。免疫组织化学显示，Small-ob小鼠胰岛中的A细胞数量增加，B细胞数量减少。这些变化随着年龄的增长而更加严重。在老年Small-ob小鼠中，发现血管周围和导管周围有细胞浸润，但在任何检查的动物中均未证实胰岛组织有炎症变化。Small-ob小鼠的糖尿病症状似乎源于胰岛A细胞数量增加导致的高胰高血糖素血症相关的胰岛素/胰高血糖素（I/G）比值差异。
