Takahashi Y, Hitomi S, Hirata T, Fukuse T, Yamazaki F, Cho K, Wada H
Department of Thoracic Surgery, Kyoto University, Japan.
Thorac Cardiovasc Surg. 1992 Oct;40(5):288-91. doi: 10.1055/s-2007-1020166.
The tracheal tissue of a rat was heterotopically transplanted beneath the abdominal skin of a syngenetic rat, and the transplanted graft was irradiated with a He-Ne laser (2.41 J/cm2). Four or seven days following transplantation, Chinese ink staining was used to ascertain the number of newly generated vessels. Rats sacrificed on day 4 were divided into 3 groups, according to the number of laser irradiation treatments: A0, A2, and A4. Similarly, rats sacrificed on day 7 were divided into groups B0, B2, and B4. Among the A groups, vascular counts were 49.5 +/- 31.1 in group A0 (n = 6), 108.9 +/- 34.7 in group A2 (n = 7), and 90.9 +/- 24.6 in group A4 (n = 4). Neovascularization was significantly more pronounced in the irradiated groups that in the non-irradiated group. In the B groups, vascular counts were 148 +/- 58 in group B0 (n = 6), 146 +/- 56 in group B2 (n = 5), and 147 +/- 49 in group B4 (n = 5). There was no significant difference between the irradiated and non-irradiated B groups. Laser irradiation was determined to stimulate neovascularization, and the possibility of a clinical application for this procedure was suggested.
将大鼠的气管组织异位移植到同基因大鼠的腹部皮肤下，并用氦氖激光（2.41 J/cm²）照射移植的移植物。移植后4天或7天，用中国墨汁染色法确定新生血管的数量。在第4天处死的大鼠根据激光照射治疗次数分为3组：A0、A2和A4。同样，在第7天处死的大鼠分为B0、B2和B4组。在A组中，A0组（n = 6）的血管计数为49.5±31.1，A2组（n = 7）为108.9±34.7，A4组（n = 4）为90.9±24.6。照射组的新生血管形成明显比未照射组更显著。在B组中，B0组（n = 6）的血管计数为148±58，B2组（n = 5）为146±56，B4组（n = 5）为147±49。照射组和未照射组的B组之间没有显著差异。确定激光照射可刺激新生血管形成，并提出了该程序临床应用的可能性。
