Schlecht S, Freudenberg M A, Galanos C
Max-Planck-Institut für Immunbiologie, Freiburg, Germany.
Zentralbl Bakteriol. 1992 Oct;277(3):288-95. doi: 10.1016/s0934-8840(11)80905-9.
Lipopolysaccharide (LPS) preparations from Salmonella S-form bacteria contain, in addition to S-form LPS, variable amounts of R-form material, lacking the O-specific polysaccharide. In the present study, we investigated the R-form LPS present in 22 LPS preparations derived from different Salmonella S-form strains belonging to 8 serological groups. The R-form part of the LPSs was separated by SDS-polyacrylamide gel electrophoresis and its antigen specificity analyzed by subsequent immunoblotting using Salmonella R-antisera (anti-Ra, -Rb1, -Rb2, -Rc). The results show that different R-determinants were present in one and the same LPS preparation. The pattern of reaction varied considerably among individual strains. This variation was also present among strains of the same serogroup. Approximately one half of the R-form LPSs exhibited a weak or no reaction with Ra antiserum. In contrast, almost all R-form LPSs showed a significant reaction with Rb1 antiserum. The electrophoretic mobility of the R-form material was very similar, exhibiting only minor differences among the different LPS preparations. In most cases, migration was similar to that of authentic Salmonella Ra-LPS; in some cases, the migration was somewhat faster resembling that of Rb1-LPS.
来自沙门氏菌S型细菌的脂多糖（LPS）制剂，除了S型LPS外，还含有数量不等的R型物质，缺少O特异性多糖。在本研究中，我们调查了来自8个血清群的不同沙门氏菌S型菌株的22种LPS制剂中存在的R型LPS。通过SDS-聚丙烯酰胺凝胶电泳分离LPS的R型部分，并使用沙门氏菌R抗血清（抗Ra、-Rb1、-Rb2、-Rc）通过后续免疫印迹分析其抗原特异性。结果表明，在同一LPS制剂中存在不同的R决定簇。各菌株之间的反应模式差异很大。同一血清群的菌株之间也存在这种差异。大约一半的R型LPS与Ra抗血清反应较弱或无反应。相比之下，几乎所有R型LPS与Rb1抗血清都有明显反应。R型物质的电泳迁移率非常相似，不同LPS制剂之间仅表现出微小差异。在大多数情况下，迁移与正宗沙门氏菌Ra-LPS相似；在某些情况下，迁移稍快，类似于Rb1-LPS。
