Mori Masanobu, Tanaka Kazuhiko, Helaleh Murad I H, Xu Qun, Ikedo Mikaru, Ogura Yutaka, Sato Shinji, Hu Wenzhi, Hasebe Kiyoshi
National Institute and Advanced Industrial Science and Technology at Seto, 110 Nishiibara-cho, Seto, Aichi 489-0884, Japan.
J Chromatogr A. 2003 May 16;997(1-2):191-7. doi: 10.1016/s0021-9673(03)00625-3.
This paper describes an ion-exclusion chromatographic system for the rapid and selective determination of ammonium ion. The optimized ion-exclusion chromatographic system was established with a polymethacrylate-based weakly basic anion-exchange resin column (TSKgel DEAE-5PW) as the separation column, an aqueous solution containing 0.05 mM tetramethylammonium hydroxide (pH 9.10) as eluent with conductimetric detection for the analyte determination. Under the optimum chromatographic conditions, ammonium ion was determined within 2.3 min with a detection limit (S/N=3) better than 0.125 microM. Ammonium ion in rain and river waters was precisely determined using this ion-exclusion chromatographic system.
本文描述了一种用于快速、选择性测定铵离子的离子排斥色谱系统。以聚甲基丙烯酸酯基弱碱性阴离子交换树脂柱（TSKgel DEAE - 5PW）作为分离柱，含0.05 mM氢氧化四甲铵（pH 9.10）的水溶液作为洗脱液，并采用电导检测法测定分析物，建立了优化的离子排斥色谱系统。在最佳色谱条件下，2.3分钟内可测定铵离子，检测限（S/N = 3）优于0.125 μM。利用该离子排斥色谱系统准确测定了雨水和河水中的铵离子。
