Benabdelmouna A, Guéritaine G, Abirached-Darmency M, Darmency H
Unité de Malherbologie et Agronomie, INRA BP 86510, 21065 Dijon CEDEX, France.
Genome. 2003 Jun;46(3):469-72. doi: 10.1139/g03-020.
Genomic in situ hybridization (GISH) applied to the F1 interspecific hybrid between oilseed rape (Brassica napus, AACC, 2n = 38) and wild radish (Raphanus raphanistrum, RrRr, 2n = 18) showed the predicted 19 chromosomes from B. napus and 9 chromosomes from R. raphanistrum. The very low female fertility of these interspecific hybrids when backcrossed to R. raphanistrum led to only two descendants. Their chromosome number varied between 45 and 48. Both of these progenies showed only 9 chromosomes from R. raphanistrum and 36-39 chromosomes from B. napus. These results indicate the efficiency and limits of GISH as a suitable tool to assess and interpret the behavior of chromosomes after such interspecific crosses. The unexpected chromosome combination is discussed.
基因组原位杂交（GISH）应用于油菜（甘蓝型油菜，AACC，2n = 38）和野生萝卜（萝卜，RrRr，2n = 18）之间的F1种间杂种，结果显示有预期的来自甘蓝型油菜的19条染色体和来自萝卜的9条染色体。这些种间杂种与萝卜回交时雌性育性极低，仅产生了两个后代。它们的染色体数在45到48之间变化。这两个后代都仅显示出来自萝卜的9条染色体和来自甘蓝型油菜的36 - 39条染色体。这些结果表明GISH作为评估和解释此类种间杂交后染色体行为的合适工具的有效性和局限性。文中还讨论了意外的染色体组合情况。
