Makino I, Murakami Y, Murakami M, Saito Y, Wada Y, Nishiyama Y
Department of Otolaryngology, Kyoto Prefectural University of Medicine.
Nihon Jibiinkoka Gakkai Kaiho. 1992 Dec;95(12):1923-30.
Cytoskeletal proteins from each epithelial component of the parotid gland were studied immunohistologically using monospecific monoclonal antibodies, and we obtained several interesting results: 1) The expression of vimentin, S-100 protein and GFAP were essentially unrecognizable. 2) The expression of alpha-actin was recognized only in myoepithelial cells, and was negative in other tissues. 3) The expression of CK-3, CK-6, CK-7, CK-8 and CK-18 was commonly recognized in both acinal and ductal cells. 4) The expression of CK-13 and CK-19 was recognized in ductal cells, but was negative in acinal cells. 5) We propose that the characteristic cell be referred to as a "duct satellite cell", as it comprises part of the intercalated duct and is sporadically localized around striated and layer ducts. 6) These duct satellite cells express all subclasses of cytokeratin observed in myoepithelial, acinal and ductal cells. 7) The duct satellite cells observed in this study are most likely to correspond to the undifferentiated reserve cells described in "bicellular theory", and we speculate that all epithelial tissues of the parotid gland originate from these cells.
利用单特异性单克隆抗体对腮腺各上皮成分的细胞骨架蛋白进行了免疫组织学研究，我们获得了一些有趣的结果：1）波形蛋白、S-100蛋白和胶质纤维酸性蛋白的表达基本无法识别。2）α-肌动蛋白的表达仅在肌上皮细胞中被识别，在其他组织中为阴性。3）CK-3、CK-6、CK-7、CK-8和CK-18的表达在腺泡细胞和导管细胞中均普遍被识别。4）CK-13和CK-19的表达在导管细胞中被识别，但在腺泡细胞中为阴性。5）我们建议将这种特征性细胞称为“导管卫星细胞”，因为它构成闰管的一部分，并散在地位于纹状管和小叶间导管周围。6）这些导管卫星细胞表达在肌上皮细胞、腺泡细胞和导管细胞中观察到的所有细胞角蛋白亚类。7）本研究中观察到的导管卫星细胞很可能与“双细胞理论”中描述的未分化储备细胞相对应，我们推测腮腺的所有上皮组织均起源于这些细胞。
