Subcellular distribution of Homer 1b/c in relation to endoplasmic reticulum and plasma membrane proteins in Purkinje neurons.

The subcellular distribution of endoplasmic reticulum proteins (IP3R1 and RYR), plasma membrane (PM) proteins (mGluR1 and PMCA Ca(2+)-pump), and scaffolding proteins, such as Homer 1b/c, was assessed by laser scanning confocal microscopy of rat cerebellum parasagittal sections. There appeared to be two classes of Ca2+ stores, nonjunctional Ca2+ stores and junctional Ca2+ stores, possibly referable to central cisternae/tubules and sub-PM cisternae, respectively, in soma, dendrites, and dendritic spines. Only some IP3R1s appeared to be part of multimeric, junctional Ca2+ signaling networks, whose composition is shown to include PMCA, mGluR1, Homer 1b/c and, not always, RYR1.