Izdebska-Szymona K, Tyski S, Drela N, Sobiczewska E, Laskowska M, Kalkhof D, Hryniewicz W
Institute of Zoology, Warsaw University, Poland.
Acta Microbiol Pol. 1992;41(1-2):49-56.
The rate of immunological and non-immunological phagocytosis of staphylococci by lipase pre-treated human granulocytes and monocytes was compared. It was found that the effect of this enzyme on two types of cells is opposite. Lipase decreases phagocytosis by granulocytes and increases by monocytes. The revealed differences between phagocytosing cells studied prompted us to investigate the influence of lipase on Fc receptors on these cells (rosette EA test). The different susceptibility of Fc receptors on non-activated phagocytes to lipase was found. This could be at least partially responsible for the difference observed between phagocytic activity of granulocytes (decreased) and monocytes (increased) pretreated with staphylococcal lipase. Inactivated enzyme showed a similar effect as active enzyme in the case of granulocytes. However, inactivated enzyme had no effect on rosette formation by lipase pretreated monocytes, indicating an enzymatic effect.
比较了脂肪酶预处理的人粒细胞和单核细胞对葡萄球菌的免疫性和非免疫性吞噬率。发现该酶对两种类型细胞的作用相反。脂肪酶降低粒细胞的吞噬作用，而增加单核细胞的吞噬作用。所研究的吞噬细胞之间显示出的差异促使我们研究脂肪酶对这些细胞上Fc受体的影响（EA玫瑰花结试验）。发现未激活的吞噬细胞上的Fc受体对脂肪酶的敏感性不同。这可能至少部分解释了用葡萄球菌脂肪酶预处理的粒细胞（降低）和单核细胞（增加）的吞噬活性之间观察到的差异。在粒细胞中，失活酶与活性酶表现出相似的作用。然而，失活酶对脂肪酶预处理的单核细胞的玫瑰花结形成没有影响，表明存在酶促作用。
