Maródi L, Leijh P C, Van Furth R
J Immunol Methods. 1983 Feb 25;57(1-3):353-61. doi: 10.1016/0022-1759(83)90095-9.
A micro-assay has been developed for the separate evaluation of phagocytosis and intracellular killing of Staphylococcus aureus by human monocytes and granulocytes. The minimal number of phagocytes required for the investigation of these functional activities of phagocytic cells has been established by performing phagocytosis and intracellular killing experiments at various cell concentrations, bacteria-to-cell ratios, and volumes. The results of these experiments revealed that phagocytosis can be measured in a reliable way, at bacteria-to-cell ratios of 5:1 and 1:1 (cell concentration 5 x 10(6)/ml), in a suspension of 200 microliters. The rate of intracellular killing by monocytes and granulocytes can also be measured with a total phagocytic suspension of 200 microliters and a cell concentration of 5 x 10(6)/ml. From these results it can be concluded that for an independent determination of the phagocytosis and intracellular killing of micro-organisms, 400 microliters of 5 x 10(6) phagocytes/ml is required, i.e., a total of 2 x 10(6) phagocytes. This number of granulocytes can be obtained from 1-2 ml of blood; for monocytes 4-8 ml of blood is required.
已开发出一种微量测定法，用于分别评估人类单核细胞和粒细胞对金黄色葡萄球菌的吞噬作用和细胞内杀伤作用。通过在不同细胞浓度、细菌与细胞比例及体积下进行吞噬作用和细胞内杀伤实验，确定了研究吞噬细胞这些功能活性所需的最少吞噬细胞数量。这些实验结果表明，在细菌与细胞比例为5:1和1:1（细胞浓度为5×10⁶/ml）、体积为200微升的悬浮液中，可以可靠地测量吞噬作用。单核细胞和粒细胞的细胞内杀伤率也可以在200微升的总吞噬悬浮液和5×10⁶/ml的细胞浓度下进行测量。从这些结果可以得出结论，为独立测定微生物的吞噬作用和细胞内杀伤作用，需要400微升5×10⁶个吞噬细胞/ml，即总共2×10⁶个吞噬细胞。这个数量的粒细胞可从1 - 2毫升血液中获得；对于单核细胞，则需要4 - 8毫升血液。
