Sephacryl S-300 Superfine的色谱特性。
Chromatographic properties of Sephacryl S-300 Superfine.
作者信息
Johansson I, Lundgren H
出版信息
J Biochem Biophys Methods. 1979;1(1):37-44. doi: 10.1016/0165-022x(79)90044-7.
Sephacryl S-300 Superfine is a new gel filtration medium that consists of allyldextran crosslinked with N,N'-methylenebisacrylamide. It was shown to have a separation range for proteins that extends to 1--2 x 10(6) dalton. The gel filtration properties were characterized by fractionation of human serum and of bovine colostrum. Thyroglobulin from pig could be isolated in one step in a very pure form. The flow properties were characterized by the relationship between flow rate and pressure drop. It was shown that Sephacryl has very good flow properties in comparison with Sephadex G-200 and that it can be used in large-scale columns.
Sephacryl S-300 Superfine是一种新型凝胶过滤介质，由烯丙基葡聚糖与N，N'-亚甲基双丙烯酰胺交联而成。它对蛋白质的分离范围可达1 - 2×10⁶道尔顿。通过对人血清和牛初乳进行分级分离来表征其凝胶过滤特性。猪甲状腺球蛋白可一步分离得到非常纯的形式。通过流速与压降之间的关系来表征其流动特性。结果表明，与Sephadex G-200相比，Sephacryl具有非常好的流动特性，可用于大型柱层析。
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