Wutzler P, Sauerbrei A, Härtl A, Reimer K
Institute for Virology and Antiviral Therapy, Friedrich Schiller University, Jena, Germany.
Dermatology. 2003;207(1):43-7. doi: 10.1159/000070940.
Due to its excellent microbicidal activity, povidone-iodine (PVP-I) is used for local anti-infective treatment, especially for wound antisepsis of the skin. Previous studies have shown that liposomal PVP-I formulations are less cytotoxic and that a special liposomal PVP-I hydrogel provides wound healing efficacy.
In the present study, the angiotoxic activity of the liposomal and the aqueous PVP-I formulations were comparatively tested at the chorioallantoic membrane (CAM) of chick embryos.
Test preparations were administered onto the CAM of ex ovo cultivated chick embryos. At different time intervals, the local irritation of the blood vessels was examined microscopically and assigned to a score of vascular injury.
Angioirritative reactions of the CAM were manifest as coagulation, vascular congestion and hemorrhages. The liposomal formulations containing 0.1, 0.5 or 1% PVP-I were found to be significantly less angiotoxic than the corresponding aqueous formulations.
The angioirritative potential of PVP-I is significantly reduced by its liposomal encapsulation.
由于具有出色的杀菌活性，聚维酮碘（PVP-I）用于局部抗感染治疗，尤其是皮肤伤口消毒。先前的研究表明，脂质体PVP-I制剂的细胞毒性较小，且一种特殊的脂质体PVP-I水凝胶具有伤口愈合功效。
在本研究中，在鸡胚绒毛尿囊膜（CAM）上对脂质体和水性PVP-I制剂的血管毒性活性进行了比较测试。
将测试制剂施用于体外培养的鸡胚的CAM上。在不同时间间隔，通过显微镜检查血管的局部刺激情况，并赋予血管损伤评分。
CAM的血管刺激性反应表现为凝血、血管充血和出血。发现含有0.1%、0.5%或1%PVP-I的脂质体制剂的血管毒性明显低于相应的水性制剂。
PVP-I的脂质体包封显著降低了其血管刺激潜力。
