Watchararot Tanapong, Prasongchean Weerapong, Thongnuek Peerapat
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Science, Chulalongkorn University, Bangkok 10330, Thailand.
Biomedical Engineering Program, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand.
R Soc Open Sci. 2021 Mar 24;8(3):201618. doi: 10.1098/rsos.201618.
Angiogenesis is a crucial step in tissue regeneration and repair. Biomaterials that allow or promote angiogenesis are thus beneficial. In this study, angiogenic properties of salt-leached silk fibroin (SF) scaffolds seeded with human adipose stem cells (hADSCs) were studied using chick chorioallantoic membrane (CAM) as a model. The hADSC-seeded SF scaffolds (SF-hADSC) with the porosity of 77.34 ± 6.96% and the pore diameter of 513.95 ± 4.99 µm were implanted on the CAM of chick embryos that were on an embryonic day 8 (E8) of development. The SF-hADSC scaffolds induced a spoke-wheel pattern of capillary network indicative of angiogenesis, which was evident since E11. Moreover, the ingrowth of blood vessels into the scaffolds was seen in histological sections. The unseeded scaffolds induced the same extent of angiogenesis later on E14. By contrast, the control group could not induce the same extent of angiogenesis. cytotoxicity tests and angioirritative study reaffirmed the biocompatibility of the scaffolds. This work highlighted that the biocompatible SF-hADSC scaffolds accelerate angiogenesis, and hence they can be a promising biomaterial for the regeneration of tissues that require angiogenesis.
血管生成是组织再生和修复的关键步骤。因此，能够允许或促进血管生成的生物材料是有益的。在本研究中，以鸡胚绒毛尿囊膜（CAM）为模型，研究了接种人脂肪干细胞（hADSCs）的盐析丝素蛋白（SF）支架的血管生成特性。将孔隙率为77.34±6.96%、孔径为513.95±4.99 µm的接种hADSCs的SF支架（SF-hADSC）植入发育至胚胎第8天（E8）的鸡胚CAM上。SF-hADSC支架诱导出毛细血管网络的辐轮状模式，表明有血管生成，自E11起这一现象就很明显。此外，在组织学切片中可见血管长入支架。未接种的支架在E14后期诱导出相同程度的血管生成。相比之下，对照组不能诱导出相同程度的血管生成。细胞毒性试验和血管刺激性研究再次证实了支架的生物相容性。这项工作强调了生物相容性的SF-hADSC支架可加速血管生成，因此它们可能是用于需要血管生成的组织再生的有前景的生物材料。
