Piskin Gamze, Tursen Umit, Bos Jan D, Teunissen Marcel B M
Department of Dermatology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
Dermatology. 2003;207(1):51-3. doi: 10.1159/000070942.
Upon a single high dose of UVB irradiation of psoriatic lesional skin, IFN-gamma expression is decreased, whereas IL-4 expression is enhanced. A similar type 1 to type 2 shift was found in dermal T cells derived from irradiated lesional skin as compared to unexposed lesional psoriatic skin. We have found recently that the IL-4 protein detected in situ upon UVB exposure of normal skin was not associated with T cells but with infiltrating neutrophils.
To determine which cell types express IL-4 in psoriatic skin after UVB irradiation.
Skin biopsies were obtained from healthy controls and psoriasis patients before and after local UVB exposure. Double immunohistochemical stainings were performed to determine the identity of IL-4-expressing cells.
In the irradiated skin of both healthy controls and patients, IL-4-positive cells coexpressed elastase and CD15, but not CD3.
IL-4-expressing cells found in psoriatic skin after a single high-dose UVB exposure appeared to be neutrophils.
对银屑病皮损皮肤进行单次高剂量紫外线B（UVB）照射后，γ干扰素表达降低，而白细胞介素-4（IL-4）表达增强。与未暴露的银屑病皮损皮肤相比，在来自照射皮损皮肤的真皮T细胞中发现了类似的从1型向2型的转变。我们最近发现，正常皮肤经UVB照射后原位检测到的IL-4蛋白与T细胞无关，而是与浸润的中性粒细胞有关。
确定UVB照射后银屑病皮肤中哪些细胞类型表达IL-4。
在局部UVB照射前后，从健康对照者和银屑病患者身上获取皮肤活检样本。进行双重免疫组织化学染色以确定表达IL-4的细胞的身份。
在健康对照者和患者的照射皮肤中，IL-4阳性细胞共表达弹性蛋白酶和CD15，但不表达CD3。
单次高剂量UVB照射后银屑病皮肤中发现的表达IL-4的细胞似乎是中性粒细胞。
