Yuan J, Murrell G A C, Wei A-Q, Appleyard R C, Del Soldato P, Wang M-X
Orthopaedic Research Institute, St George Hospital Campus, University of New South Wales, 4-10 South Street, Kogarah, Sydney, NSW 2217, Australia.
Inflamm Res. 2003 Jun;52(6):230-7. doi: 10.1007/s00011-003-1167-7.
To determine if the addition of nitric oxide (NO) via nitroflurbiprofen (NO-flurbiprofen) would enhance rat Achilles tendon healing.
Sixty-five male Sprague-Dawley rats were randomly divided into NO-flurbiprofen, flurbiprofen and vehicle groups, given drugs or vehicle subcutaneously, and their right Achilles tendon divided. Histological assessment was carried out at day 5, 10, and 15 post-operation. Healing tendon biomechanical properties and hydroxyproline content were measured at day 10.
The healing Achilles tendon from the NO-flurbiprofen and flurbiprofen groups showed a better organization of extracellular collagenous matrix than that from the vehicle group. Flurbiprofen and NO-flurbiprofen decreased healing tendon cross-sectional area by 30% and 20%. This reduction was accompanied by a decreased failure load in the flurbiprofen group, but not the NO-flurbiprofen group. NO-flubiprofen prevented the reduction of body weight gain observed in the flubiprofen group.
Both flurbiprofen and NO-flurbiprofen promoted better collagen reorganization during tendon healing. NO-flurbiprofen further improved tendon healing by increasing tendon stress and reducing the side effects (body weight loss) of flurbiprofen. The enhanced tendon healing by NO-flurbiprofen is likely due to the release of NO from the compound.
确定通过氟比洛芬硝化物（一氧化氮 - 氟比洛芬）添加一氧化氮（NO）是否会促进大鼠跟腱愈合。
65只雄性Sprague - Dawley大鼠随机分为一氧化氮 - 氟比洛芬组、氟比洛芬组和赋形剂组，皮下给予药物或赋形剂，然后切断其右跟腱。在术后第5、10和15天进行组织学评估。在第10天测量愈合跟腱的生物力学性能和羟脯氨酸含量。
一氧化氮 - 氟比洛芬组和氟比洛芬组愈合的跟腱与赋形剂组相比，细胞外胶原基质组织更佳。氟比洛芬和一氧化氮 - 氟比洛芬使愈合跟腱的横截面积分别减少30%和20%。这种减少在氟比洛芬组伴随着破坏载荷降低，但在一氧化氮 - 氟比洛芬组未出现。一氧化氮 - 氟比洛芬防止了氟比洛芬组观察到的体重增加减少。
氟比洛芬和一氧化氮 - 氟比洛芬在肌腱愈合过程中均促进了更好的胶原重组。一氧化氮 - 氟比洛芬通过增加肌腱应力和减少氟比洛芬的副作用（体重减轻）进一步改善了肌腱愈合。一氧化氮 - 氟比洛芬增强肌腱愈合可能是由于该化合物释放一氧化氮所致。
