Alilou Hakim, Akssira Mohamed
Sultan Moulay Slimane University of Beni Mellal, Research Group in Environmental Sciences and Applied Materials (SEMA), FP Khouribga, B.P. 145, 25000 Khouribga, Morocco.
Laboratory of Bio-organic and Analytic Chemistry, Faculty of Science and Technology, Hassan II University, BP: 146, Mohammadia 20650, Morocco.
Saudi J Biol Sci. 2021 Dec;28(12):6756-6764. doi: 10.1016/j.sjbs.2021.07.052. Epub 2021 Jul 24.
The use of chemical products to neutralize microorganisms has always been a subject of discussion and research for alternative solutions, indeed, the use of essential oils has been a promising natural methodology.
In our study we used the essential oils from different parts of (Apiaceae), obtained by hydrodistillation, were identified and using Gas chromatography-mass spectrometry (GC-MS) and Gas Chromatography-Flame Ionization Detection (GC/FID) methods and evaluated against several bacteria of Gram- and Gram + bacteria. Disk diffusion, Minimum Inhibitory Concentration (MIC) and Minimum Microbicidal Concentration (MMC) methods have been used. Free radical-scavenging activity and insecticidal activity of essential oils were also identified.
Majority products from different parts of essential oil identified by GC-MS and GC/FID methods are 2,6-Dimethylnaphthalene, Pinane and Hexahydrofarnesyl acetone. The highest activity was found against using inflorescence essential oil with minimal inhibitory concentration value for 0,56 μg/μL. Insecticidal activity was also the subject of this study, roots and inflorescence essential oils demonstrated to have a remarkable potent against and using contact assessment, inhalation assessment and ingestion assessment tests. Insecticidal activity assay results showed a significant enhancement of mortality in both test insect pest on increasing the dose and exposure period. In the other hand, the different essential oils of were evaluated for their radical scavenging activities by means of the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay. The strongest scavenging activity was observed in inflorescences essential oil fraction scavenged radicals effectively at 100% using 500 mgL concentration.
Its essential oils were proved to have strong antimicrobial, insecticidal and antioxidant activities that allows it to be used by the pharmaceutical and cosmetic industries as natural preservative.
使用化学产品来中和微生物一直是讨论和研究替代解决方案的主题，事实上，使用精油一直是一种有前景的天然方法。
在我们的研究中，我们使用了通过水蒸馏从（伞形科）不同部位获得的精油，通过气相色谱 - 质谱联用（GC - MS）和气相色谱 - 火焰离子化检测（GC/FID）方法进行鉴定，并针对几种革兰氏阴性和革兰氏阳性细菌进行评估。使用了纸片扩散法、最低抑菌浓度（MIC）和最低杀菌浓度（MMC）方法。还鉴定了精油的自由基清除活性和杀虫活性。
通过GC - MS和GC/FID方法鉴定的来自不同部位的大多数精油产品为2,6 - 二甲基萘、蒎烷和六氢法呢基丙酮。发现使用花序精油对[具体细菌名称未给出]具有最高活性，其最低抑菌浓度值为0.56μg/μL。杀虫活性也是本研究的主题，根和花序精油通过接触评估、吸入评估和摄入评估测试对[具体害虫名称未给出]表现出显著的效力。杀虫活性测定结果表明，随着剂量和暴露时间的增加，两种测试害虫的死亡率显著提高。另一方面，通过2,2 - 二苯基 - 1 - 苦基肼（DPPH）测定法评估了[植物名称未给出]的不同精油的自由基清除活性。在花序精油馏分中观察到最强的清除活性，在500mg/L浓度下能有效清除100%的自由基。
其精油被证明具有强大的抗菌、杀虫和抗氧化活性，这使得它可以被制药和化妆品行业用作天然防腐剂。
