Zimnoch-Guzowska E, Lebecka R, Kryszczuk A, Maciejewska U, Szczerbakowa A, Wielgat B
Plant Breeding and Acclimatization Institute (IHAR), Młochów Research Center, 05-831 Młochów, Poland.
Theor Appl Genet. 2003 Jun;107(1):43-8. doi: 10.1007/s00122-003-1221-4. Epub 2003 Mar 27.
In breeding for resistance to late blight, ( Phytophthora infestans Mont. de Bary), an economically important disease affecting potatoes, the search for new sources of durable resistance includes the non-host wild Solanum species. The aim of this work was to evaluate the resistance to P. infestans in the somatic hybrids between S. nigrum L. and diploid potato clone ZEL-1136. Sixteen somatic hybrids, their fusion parents, and three standard potato cultivars were screened for resistance to P. infestans in two types of tests-on whole plants and detached leaves-with two highly aggressive and virulent isolates of P. infestans, US8 and MP322. In the whole plant assay, the foliage of the somatic hybrids showed no symptoms of infection, while the foliage of the potato fusion parent and the standard cultivars was infected with P. infestans. In the detached leaflet assay, the breaking-down of resistance of the S. nigrum L. parent and the variable response of individual hybrid clones were noted. Nine S. nigrum L. (+) ZEL-1136 hybrids showed a resistance that was significantly higher than that of S. nigrum, while six clones expressed a resistance to P. infestans similar to that of S. nigrum. The results confirm the effective transfer of late blight resistance of S. nigrum into its somatic hybrids with potato.
在培育抗晚疫病（致病疫霉，蒙氏疫霉，德巴里）的马铃薯品种时，晚疫病是一种影响马铃薯的具有重要经济意义的病害，寻找新的持久抗性来源包括非寄主野生茄属物种。这项工作的目的是评估黑茄（Solanum nigrum L.）与二倍体马铃薯克隆ZEL - 1136之间体细胞杂种对致病疫霉的抗性。在两种试验中，即对整株植物和离体叶片进行试验，用两种高侵袭性和毒性的致病疫霉分离株US8和MP322，对16个体细胞杂种、它们的融合亲本以及三个标准马铃薯品种进行了抗致病疫霉筛选。在整株植物试验中，体细胞杂种的叶片未表现出感染症状，而马铃薯融合亲本和标准品种的叶片被致病疫霉感染。在离体小叶试验中，观察到黑茄亲本抗性的丧失以及各个杂种克隆的不同反应。九个黑茄（+）ZEL - 1136杂种表现出的抗性显著高于黑茄，而六个克隆对致病疫霉的抗性与黑茄相似。结果证实了黑茄的晚疫病抗性有效地转移到了其与马铃薯的体细胞杂种中。
