Vilanova S, Romero C, Abernathy D, Abbott A G, Burgos L, Llacer G, Badenes M L
Instituto Valenciano de Investigaciones Agrarias, Moncada, Apartado Oficial, 46113 Valencia, Spain.
Mol Genet Genomics. 2003 Aug;269(5):685-91. doi: 10.1007/s00438-003-0874-7. Epub 2003 Jun 27.
To facilitate gene discovery in the Rosaceae, a bacterial artificial chromosome (BAC) library was constructed using high-molecular-weight (HMW) DNA from apricot leaves (Prunus armeniaca L.). The library contains 101,376 clones (264, 384-well plates) with an average insert size of 64 kb, equivalent to 22-fold genome coverage. In the first application of this library, high-density filters were screened for self-incompatibility genes using apricot DNA probes. Eight positive BAC clones were detected and fingerprinted to determine clone relationships and assemble contigs. These results demonstrate the suitability of this library for gene identification and physical mapping of the apricot genome.
为便于在蔷薇科中进行基因发现，利用来自杏叶（Prunus armeniaca L.）的高分子量（HMW）DNA构建了一个细菌人工染色体（BAC）文库。该文库包含101,376个克隆（264个384孔板），平均插入片段大小为64 kb，相当于基因组覆盖22倍。在该文库的首次应用中，使用杏DNA探针筛选高密度滤膜以寻找自交不亲和基因。检测到8个阳性BAC克隆并进行指纹分析，以确定克隆关系并组装重叠群。这些结果证明了该文库适用于杏基因组的基因鉴定和物理图谱构建。
