Komatsu H, Awano H, Ishibashi H, Ikeda I
Chemical Synthesis Laboratory, Mitsui Chemicals Inc., 1144 Togo, Mobarashi, Chiba 297-0017, Japan.
Nucleic Acids Res Suppl. 2001(1):49-50. doi: 10.1093/nass/1.1.49.
Manufacturing method for 2'-deoxynucleosides (dNus) has been developed using novel chemo-enzymatic process. The method consists of the chemical synthesis of 2'-deoxyribose 1-phosphate (dRP) and the enzymatic conversions of dRP into 2'-deoxyadenosine (dA), 2'-deoxyguanosine (dG) and 2'-deoxycytidine (dC). Large-scale preparation is successfully done, which demonstrates an applicability of this strategy to practical use. As thymidine is already under commercial-scale production, all four dNus are now ready for scale-up.
已开发出一种使用新型化学酶法制备2'-脱氧核苷（dNus）的方法。该方法包括2'-脱氧核糖1-磷酸（dRP）的化学合成以及将dRP酶促转化为2'-脱氧腺苷（dA）、2'-脱氧鸟苷（dG）和2'-脱氧胞苷（dC）。已成功完成大规模制备，这证明了该策略在实际应用中的适用性。由于胸苷已在商业规模生产，现在所有四种dNus都已准备好进行扩大规模生产。
