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2-脱氧-β-D-核糖1-磷酸的合成、核磁共振比较及其对合成α-异构体结构解析的酶活性

Synthesis of 2-deoxy-beta-D-ribose 1-phosphate, NMR comparison and its enzymatic activity for structural elucidation of synthetic alpha-isomer.

作者信息

Komatsu Hironori, Ikeda Ichirou

机构信息

Catalysis Science Laboratory, Mitsui Chemicals Inc., Sodegaura-shi, Chiba, Japan.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1685-6. doi: 10.1081/NCN-120023113.

DOI:10.1081/NCN-120023113
PMID:14565495
Abstract

2-Deoxy-beta-D-ribose 1-phosphate (1) was synthesized in a stereoselective manner and isolated with no detectable contamination by its alpha-isomer (4). Explicit configuration of 4 was first determined by NMR comparison with 1 judging from NOE results and their coupling constants. Natural purine nucleoside phosphorylase (PNPase) did not recognize 1 and gave no products such as alpha- or beta-deoxynucleosides.

摘要

以立体选择性方式合成了2-脱氧-β-D-核糖1-磷酸（1），并将其分离出来，未检测到其α-异构体（4）的污染。通过与1进行NMR比较，根据NOE结果及其耦合常数，首次确定了4的明确构型。天然嘌呤核苷磷酸化酶（PNPase）不识别1，也不产生α-或β-脱氧核苷等产物。

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