一种构建根癌农杆菌无Ti质粒菌株的新方法。
A new method for construction of Ti plasmid-less strains in Agrobacterium tumefaciens.
作者信息
Uraji M, Suzuki K, Yoshida K
机构信息
Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan.
出版信息
Nucleic Acids Res Suppl. 2001(1):173-4. doi: 10.1093/nass/1.1.173.
PMID:12836320
Abstract
Agrobacterium tumefaciens harboring a Ti plasmid causes plant tumorigenesis and it's DNA transfer system have been extensively used in plant biotechnology. Because Ti plasmids are high stable in agrobacterial hosts, their curing is very difficult by conventional methods. Here, we introduced a novel curing method by using Ti plasmids incompatibility.
摘要
携带Ti质粒的根癌土壤杆菌会引发植物肿瘤形成，其DNA转移系统已在植物生物技术中得到广泛应用。由于Ti质粒在农杆菌宿主中高度稳定，用传统方法消除它们非常困难。在此，我们介绍了一种利用Ti质粒不相容性的新型消除方法。
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