根癌土壤杆菌（一种细菌）与酿酒酵母（一种酵母）之间的跨界接合作用。
Trans-kingdom conjugation between Agrobacterium tumefaciens and Saccharomyces cerevisiae, a bacterium and a yeast.
作者信息
Sawasaki Y, Inomata K, Yoshida K
机构信息
Department of Biological Science, Hiroshima University, Japan. 515582/ue.ipc.hiroshima-u.ac.jp
出版信息
Plant Cell Physiol. 1996 Jan;37(1):103-6. doi: 10.1093/oxfordjournals.pcp.a028906.
For conjugation between prokaryotic Agrobacterium tumefaciens and eukaryotic Saccharomyces cerevisiae, we constructed two novel conjugative plasmids. A. tumefaciens transmitted the plasmids to S. cerevisiae with the aid of tra genes on a helper plasmid. The transmitted plasmids retained their original structure and function in transconjugant yeasts. The presence of Ti plasmid barely affected the trans-kingdom conjugation.
为了实现原核生物根癌土壤杆菌与真核生物酿酒酵母之间的接合作用，我们构建了两种新型接合质粒。根癌土壤杆菌借助辅助质粒上的tra基因将质粒传递给酿酒酵母。传递的质粒在接合后的酵母中保留了其原始结构和功能。Ti质粒的存在对跨域接合几乎没有影响。
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