含嘧啶/嘌呤混合序列的肽核酸的构象与识别控制

Conformation and recognition control of peptide ribonucleic acid containing pyrimidine/purine mixed sequence.

作者信息

Sato H, Wada T, Inoue Y

机构信息

Department of Molecular Chemistry, Osaka University, 2-1 Yamada-oka, Suita 565-0871, Japan.

出版信息

Nucleic Acids Res Suppl. 2001(1):229-30. doi: 10.1093/nass/1.1.229.

DOI:10.1093/nass/1.1.229

PMID:12836348

Abstract

Peptide ribonucleic acids (PRNA), tethering 5'-amino-5'-deoxyribonucleoside as a recognition moiety for RNA/DNA, have been designed and synthesized, as a novel nucleic acid model. External switching of the recognition behavior of PRNA containing not only pyrimidine nucleobase but also purine nucleobase, with complementary oligomeric DNA/RNA has been demonstrated for the first time through both the orientational switching of the nucleobase of PRNA and electrostatic repulsion between borate ester anion of PRNA and phosphodiester anion of DNA/RNA, induced by added borates.

摘要

作为一种新型核酸模型，已设计并合成了肽核酸（PRNA），它将5'-氨基-5'-脱氧核糖核苷作为RNA/DNA的识别部分。首次通过PRNA核苷酸碱基的取向转换以及添加硼酸盐诱导的PRNA硼酸酯阴离子与DNA/RNA磷酸二酯阴离子之间的静电排斥，证明了不仅含有嘧啶核苷酸碱基而且含有嘌呤核苷酸碱基的PRNA与互补寡聚DNA/RNA的识别行为的外部转换。