γ-PRNA的DNA识别控制及错配碱基对复合物稳定性的影响

DNA recognition control of gamma-PRNA and mismatched base effects upon complex stability.

作者信息

Sato Hirofumi, Hashimoto Yusuke, Kikkawa Mayuko, Wada Takehiko, Inoue Yoshihisa

机构信息

Department of Molecular Chemistry, Graduate School of Engineering, Osaka University, Yamada-oka, Suita 565-0871, Japan.

出版信息

Nucleic Acids Res Suppl. 2002(2):159-60. doi: 10.1093/nass/2.1.159.

DOI:10.1093/nass/2.1.159

PMID:12903154

Abstract

Oligomeric peptide ribonucleic acids (PRNA's), tethering 5'-amino-5'-deoxyribonucleosides as a recognition site for nucleic acids, has been designed and synthesized by solid phase method. PRNA 12-mer found to form stable duplex with complementary DNA.PRNA.DNA duplex composed of PRNA with mismatched DNA has 10-degree lower melting temperature than fullmatched PRNA.DNA duplex. These results demonstrate that binding of PRNA with DNA is sequence specific. It should be emphasized that the on-off switching of PRNA.DNA duplex formation has been realized by the borate added as an external factor.

摘要

寡聚肽核糖核酸（PRNA），其连接5'-氨基-5'-脱氧核糖核苷作为核酸的识别位点，已通过固相法设计并合成。发现PRNA 12聚体与互补DNA形成稳定的双链体。由带有错配DNA的PRNA组成的PRNA·DNA双链体的解链温度比完全匹配的PRNA·DNA双链体低10度。这些结果表明PRNA与DNA的结合具有序列特异性。应该强调的是，通过添加硼酸盐作为外部因素，实现了PRNA·DNA双链体形成的开关控制。