Sato Hirofumi, Hashimoto Yusuke, Kikkawa Mayuko, Wada Takehiko, Inoue Yoshihisa
Department of Molecular Chemistry, Graduate School of Engineering, Osaka University, Yamada-oka, Suita 565-0871, Japan.
Nucleic Acids Res Suppl. 2002(2):159-60. doi: 10.1093/nass/2.1.159.
Oligomeric peptide ribonucleic acids (PRNA's), tethering 5'-amino-5'-deoxyribonucleosides as a recognition site for nucleic acids, has been designed and synthesized by solid phase method. PRNA 12-mer found to form stable duplex with complementary DNA.PRNA.DNA duplex composed of PRNA with mismatched DNA has 10-degree lower melting temperature than fullmatched PRNA.DNA duplex. These results demonstrate that binding of PRNA with DNA is sequence specific. It should be emphasized that the on-off switching of PRNA.DNA duplex formation has been realized by the borate added as an external factor.
寡聚肽核糖核酸（PRNA），其连接5'-氨基-5'-脱氧核糖核苷作为核酸的识别位点，已通过固相法设计并合成。发现PRNA 12聚体与互补DNA形成稳定的双链体。由带有错配DNA的PRNA组成的PRNA·DNA双链体的解链温度比完全匹配的PRNA·DNA双链体低10度。这些结果表明PRNA与DNA的结合具有序列特异性。应该强调的是，通过添加硼酸盐作为外部因素，实现了PRNA·DNA双链体形成的开关控制。
