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兔气管培养及完整上皮中内皮素、血管加压素和P物质样免疫反应性

Endothelin, vasopressin, and substance P like immunoreactivity in cultured and intact epithelium from rabbit trachea.

作者信息

Rennick R E, Loesch A, Burnstock G

机构信息

Department of Anatomy and Developmental Biology, University College, London.

出版信息

Thorax. 1992 Dec;47(12):1044-9. doi: 10.1136/thx.47.12.1044.

DOI:10.1136/thx.47.12.1044
PMID:1283645
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1021098/
Abstract

BACKGROUND

The control of airways reactivity is essential to our understanding of disease processes such as asthma. Many studies have examined the neural control of the airways, but more recently there has been evidence to show that the epithelium lining the airways may influence airways reactivity.

METHODS

Rabbit tracheal epithelial cells were dispersed with enzymes, grown in primary culture and fixed. Tissue from intact tracheas was also sampled and fixed. Localisation of the vasoactive substances endothelin-1, arginine-vasopressin, and substance P was investigated by immunolabelling techniques.

RESULTS

Scattered immunolabelling to endothelin-1, arginine-vasopressin, and substance P was found throughout the cultures (with < 20% of cells staining positively to each antibody). At the ultrastructural level this immunoreactivity was found in the cytoplasmic matrix. In addition, immunoreactivity of intact tissue to endothelin-1, arginine-vasopressin, and substance P was examined and positively staining cells were found to be scattered through the epithelium.

CONCLUSIONS

The presence of these vasoactive substances within the epithelium lining the airways supports the view that epithelial cells may provide an additional mechanism in the control of airways reactivity.

摘要

背景

气道反应性的控制对于我们理解诸如哮喘等疾病过程至关重要。许多研究已经探讨了气道的神经控制，但最近有证据表明，气道内衬的上皮细胞可能会影响气道反应性。

方法

用酶分散兔气管上皮细胞，进行原代培养并固定。完整气管的组织也进行取样并固定。采用免疫标记技术研究血管活性物质内皮素 -1、精氨酸加压素和P物质的定位。

结果

在整个培养物中发现了对内皮素 -1、精氨酸加压素和P物质的散在免疫标记（每种抗体染色阳性的细胞<20%）。在超微结构水平上，这种免疫反应性存在于细胞质基质中。此外，检查了完整组织对内皮素 -1、精氨酸加压素和P物质的免疫反应性，发现阳性染色细胞散在于上皮中。

结论

气道内衬上皮内存在这些血管活性物质支持了上皮细胞可能在气道反应性控制中提供额外机制的观点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f53/1021098/183f3baaa7e6/thorax00372-0056-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f53/1021098/9cea813d5557/thorax00372-0053-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f53/1021098/e203b5c20a45/thorax00372-0054-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f53/1021098/7f74247ceac1/thorax00372-0055-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f53/1021098/183f3baaa7e6/thorax00372-0056-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f53/1021098/9cea813d5557/thorax00372-0053-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f53/1021098/e203b5c20a45/thorax00372-0054-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f53/1021098/7f74247ceac1/thorax00372-0055-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f53/1021098/183f3baaa7e6/thorax00372-0056-a.jpg

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