Loesch A, Bodin P, Burnstock G
Department of Anatomy and Developmental Biology, University College London, UK.
Peptides. 1991 Sep-Oct;12(5):1095-103. doi: 10.1016/0196-9781(91)90065-w.
The localization and colocalization of endothelin-1, arginine-vasopressin and serotonin in the endothelial cells of rabbit aorta in primary culture were investigated by preembedding and postembedding immunocytochemistry. These three substances were localized in the same population of cells, where they appeared in high proportions (greater than 60%). These findings indicate: 1) that the cell population is heterogeneous, and 2) that these substances are colocalized in some of the cultured endothelial cells. Double labeling in single cells has demonstrated the simultaneous presence of 1) endothelin and vasopressin, 2) vasopressin and serotonin, and 3) endothelin and serotonin. The immunolabeling dominated in the cytoplasmic matrix.
采用包埋前和包埋后免疫细胞化学方法，研究了原代培养的兔主动脉内皮细胞中内皮素 -1、精氨酸加压素和5-羟色胺的定位及共定位情况。这三种物质定位于同一细胞群体中，且在该群体中占比很高（大于60%）。这些发现表明：1）该细胞群体是异质性的；2）这些物质在一些培养的内皮细胞中共定位。单细胞双标记已证实1）内皮素和加压素、2）加压素和5-羟色胺、3）内皮素和5-羟色胺同时存在。免疫标记主要出现在细胞质基质中。
